Desde sra hasta blastx

Annotation:

StepAnnotation
Step 1: Bowtie2
Paired-end Dataset Collection
select at runtime
True
False
No
Use a built-in genome index
Vitis_vinifera_JGI_12xMarch2010
Do not set
1: Default setting only
No, just use defaults
No
True
Step 2: Input dataset
select at runtime
Step 3: Input dataset
select at runtime
Step 4: rnaviralSPAdes
Paired-end: interlaced reads
Output dataset 'output_unaligned_reads_l' from step 1,Output dataset 'output_unaligned_reads_r' from step 1
Default (--pe)
FR (-> <-)
Disabled
Additional read files:
select at runtime
select at runtime
select at runtime
select at runtime
select at runtime
Nothing selected.
Auto
Auto
Assembly graph Assembly graph with scaffolds Contigs Scaffolds
Step 5: NCBI BLAST+ blastn
Output dataset 'out_cn' from step 4
FASTA file from your history (see warning note below)
Empty.
Empty.
Output dataset 'output' from step 2
megablast - Traditional megablast used to find very similar (e.g., intraspecies or closely related species) sequences
0.001
Tabular (standard 12 columns)
Hide Advanced Options
Step 6: Filter sequences by ID
Output dataset 'out_cn' from step 4
tabular file
Output dataset 'output1' from step 5
1
Both positive matches (ID on list) and negative matches (ID not on list), as two files
Show Advanced Options
False
Step 7: NCBI BLAST+ blastx
Output dataset 'output_neg' from step 6
FASTA file from your history (see warning note below)
Empty.
Empty.
Output dataset 'output' from step 3
1. Standard
blastx - Traditional BLASTX to compare translated nucleotide query to protein database
0.001
Tabular (extended 25 columns)
Show Advanced Options
True
Both
Use Defaults
0
Not available.
Not available.
False
False
No restriction, search the entire database
0.0
Not available.
Not available.
Use default