##FastQC 0.11.8 >>Basic Statistics pass #Measure Value Filename NGS187-5_S5_L001_R2_001_fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 885950 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.684001354478244 33.0 32.0 33.0 29.0 34.0 2 32.00157909588577 33.0 32.0 33.0 30.0 34.0 3 32.02487499294543 33.0 32.0 33.0 31.0 34.0 4 31.837057396015577 33.0 32.0 34.0 30.0 34.0 5 32.2699125232801 33.0 32.0 34.0 32.0 34.0 6 34.89801456064112 37.0 33.0 37.0 32.0 37.0 7 35.13157063039675 37.0 34.0 37.0 32.0 37.0 8 35.09735989615667 37.0 34.0 37.0 32.0 37.0 9 35.07584175179186 37.0 34.0 37.0 33.0 37.0 10-14 35.8057608217168 37.4 35.2 37.4 34.2 37.4 15-19 37.06997189457644 38.0 38.0 38.0 36.0 38.0 20-24 37.24695908346972 38.4 38.0 38.4 36.0 38.4 25-29 37.55974400361194 38.8 38.0 39.0 36.0 39.0 30-34 37.503541283368136 38.4 38.0 39.0 36.0 39.0 35-39 37.47573000733676 38.4 38.0 39.0 36.0 39.0 40-44 37.47095118234664 38.6 38.0 39.0 36.0 39.0 45-49 37.37745538687285 38.0 38.0 39.0 36.0 39.0 50-54 37.374435351882155 38.0 38.0 39.0 36.0 39.0 55-59 37.35286009368475 38.0 38.0 39.0 36.0 39.0 60-64 37.364702071222986 38.0 38.0 39.0 36.0 39.0 65-69 37.32415373328065 38.0 38.0 39.0 36.0 39.0 70-74 37.19253930808737 38.0 38.0 39.0 35.8 39.0 75-79 37.28332772729838 38.0 38.0 39.0 36.0 39.0 80-84 37.30015463626616 38.0 38.0 39.0 36.0 39.0 85-89 37.27939838591343 38.0 38.0 39.0 36.0 39.0 90-94 37.19207607652802 38.0 38.0 39.0 35.8 39.0 95-99 37.17189570517523 38.0 38.0 39.0 35.2 39.0 100-104 37.11842496754896 38.0 38.0 39.0 35.0 39.0 105-109 37.05110220667081 38.0 38.0 39.0 34.4 39.0 110-114 36.97521553135052 38.0 38.0 39.0 34.0 39.0 115-119 36.923696371127036 38.0 38.0 39.0 34.0 39.0 120-124 36.82796094587731 38.0 38.0 39.0 34.0 39.0 125-129 36.713932163214636 38.0 38.0 39.0 34.0 39.0 130-134 36.62753563970879 38.0 37.8 39.0 34.0 39.0 135-139 36.508663468593035 38.0 37.0 39.0 33.2 39.0 140-144 36.36347626841244 38.0 37.0 39.0 33.0 39.0 145-149 36.25151916022349 38.0 37.0 39.0 32.6 39.0 150-151 34.943364749703704 37.5 35.0 38.5 24.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3248336672444978 1101 2 -0.1503179109550139 1101 3 -0.18981858332913504 1101 4 -0.02946286042578805 1101 5 0.03981814062576916 1101 6 -0.031427662103745035 1101 7 -0.07175568683815214 1101 8 -0.0729748250496769 1101 9 -0.07644799678003977 1101 10-14 -0.10512614119534192 1101 15-19 0.008672861859778891 1101 20-24 0.0498284801732467 1101 25-29 0.0497483405063619 1101 30-34 -0.036005676116644736 1101 35-39 0.00886214849818856 1101 40-44 0.0491264235745561 1101 45-49 -0.057377437830446354 1101 50-54 -0.12293777336199696 1101 55-59 -0.07942930970712325 1101 60-64 -0.08039141060123 1101 65-69 -0.061657614482378165 1101 70-74 0.11457135713528288 1101 75-79 0.00886799574797692 1101 80-84 -0.004861902360900672 1101 85-89 -0.0837159780795318 1101 90-94 -0.004860608266952227 1101 95-99 0.03149395094420271 1101 100-104 8.249182561996804E-4 1101 105-109 0.009725945653691781 1101 110-114 0.02619435109137669 1101 115-119 0.006663713520381975 1101 120-124 0.06463702400494498 1101 125-129 0.006258977752274575 1101 130-134 0.1443053062488886 1101 135-139 0.05429303106559047 1101 140-144 0.10837273705798367 1101 145-149 -0.03299211370153188 1101 150-151 0.12465370225854855 1102 1 -0.20168678331465983 1102 2 -0.10457787071556623 1102 3 -0.12641596254239928 1102 4 -0.31217979407049157 1102 5 -0.08090579637387663 1102 6 -0.17207236370263246 1102 7 0.0013256649884141325 1102 8 -0.45746454508956447 1102 9 0.03917637370141591 1102 10-14 -0.014938236904548319 1102 15-19 0.04834782511454705 1102 20-24 -0.04765904137027377 1102 25-29 0.007379535279710581 1102 30-34 0.06567341304928931 1102 35-39 0.11485099933617704 1102 40-44 0.05358826449195675 1102 45-49 -0.015410280978677804 1102 50-54 -0.02293764901265405 1102 55-59 -0.07916655954733187 1102 60-64 -0.03186501247940754 1102 65-69 0.04548065949902025 1102 70-74 0.02579954315195465 1102 75-79 -0.04825268280151107 1102 80-84 -0.12927764697719368 1102 85-89 -0.22414418747387543 1102 90-94 -0.13165011964864703 1102 95-99 -0.21221534018994248 1102 100-104 -0.28588226142611717 1102 105-109 -0.1911811206220193 1102 110-114 -0.12638291337231777 1102 115-119 -0.1474193593010824 1102 120-124 -0.10646499716926172 1102 125-129 -0.20261993831937986 1102 130-134 -0.3252277992764476 1102 135-139 -0.2879364407232714 1102 140-144 -0.2732805744893483 1102 145-149 -0.03585451120657979 1102 150-151 -0.08886375514558864 1103 1 -0.2490504507522786 1103 2 -0.3648701256803939 1103 3 -0.24284868219103828 1103 4 -0.14598800741553575 1103 5 -0.32742653099911934 1103 6 -0.21444542478251094 1103 7 -0.167153197029414 1103 8 -0.11375527062472202 1103 9 0.026219260106955744 1103 10-14 -0.008676993849974224 1103 15-19 0.08394891841974328 1103 20-24 -0.10683487089250576 1103 25-29 -0.04554704793781639 1103 30-34 0.0695223113858745 1103 35-39 0.07282546975348225 1103 40-44 0.06130995070687817 1103 45-49 -0.09464347035100928 1103 50-54 -0.10376135375871343 1103 55-59 -0.09974695274132728 1103 60-64 -0.0443165368775027 1103 65-69 0.06033421598437627 1103 70-74 0.22292001131211947 1103 75-79 -0.0511141675375697 1103 80-84 -0.0010105598213243638 1103 85-89 0.008952537517373571 1103 90-94 -0.03925304427745857 1103 95-99 -0.07962900725330968 1103 100-104 -0.15301287172594158 1103 105-109 -0.13392637202981206 1103 110-114 -0.0819116477713493 1103 115-119 -0.09464993974417268 1103 120-124 -0.10768535979129723 1103 125-129 -0.13680493693465934 1103 130-134 -0.3184614868153872 1103 135-139 -0.1902365939989039 1103 140-144 -0.23650421552996193 1103 145-149 -0.19907821462197717 1103 150-151 -0.11980965209080097 1104 1 -0.2767687749664205 1104 2 -0.6325345393699102 1104 3 -0.3758668669351657 1104 4 -0.2968945625061892 1104 5 -0.29348033423911346 1104 6 -0.27550110550346574 1104 7 -0.2394415781044117 1104 8 -0.21178525667772163 1104 9 -0.019395480800675102 1104 10-14 -0.09892750345195367 1104 15-19 -0.03793124465288855 1104 20-24 -0.09531706164457177 1104 25-29 -0.20800011091390047 1104 30-34 -0.1313078034086672 1104 35-39 -0.026785355265289468 1104 40-44 -0.08033391797673772 1104 45-49 -0.17805190292262552 1104 50-54 -0.08803852359027786 1104 55-59 -0.10725043949167912 1104 60-64 -0.08917518938260827 1104 65-69 -0.11227575075738372 1104 70-74 0.0684650172127732 1104 75-79 -0.17108412784224925 1104 80-84 -0.0331406960727989 1104 85-89 -0.046849415062830246 1104 90-94 -4.127910857434358E-4 1104 95-99 -0.08959011657633198 1104 100-104 -0.0070301445507467974 1104 105-109 0.00525746017702744 1104 110-114 0.02697178748238116 1104 115-119 0.030392705894712435 1104 120-124 0.011163207958944099 1104 125-129 -0.034936575170902984 1104 130-134 -0.42200080663085515 1104 135-139 -0.069967846010492 1104 140-144 -0.14669142720252637 1104 145-149 -0.23728414746179993 1104 150-151 -0.32426799883470636 1105 1 -0.2352003250045982 1105 2 -0.2976223494144925 1105 3 -0.23398806450160592 1105 4 -0.038996584723488326 1105 5 -0.5989210470645538 1105 6 -0.3714911545194539 1105 7 -0.3670998948117159 1105 8 -0.14379682100196334 1105 9 -0.5401022716083261 1105 10-14 -0.19808819706509695 1105 15-19 -0.13888065227376956 1105 20-24 -0.06618438596291298 1105 25-29 -0.16112272477137424 1105 30-34 -0.1122417549838346 1105 35-39 -0.051989732220391716 1105 40-44 -0.11758268026152763 1105 45-49 -0.16323996174180166 1105 50-54 -0.13204077734036446 1105 55-59 -0.23018302403809798 1105 60-64 -0.1656974559408937 1105 65-69 -0.17515654831398564 1105 70-74 -0.058628630414013116 1105 75-79 -0.21281027873267533 1105 80-84 -0.18733377964628062 1105 85-89 -0.11309562802374273 1105 90-94 -0.19225914198108285 1105 95-99 -0.11655746798893318 1105 100-104 -0.08486691392929657 1105 105-109 -0.13650631479279696 1105 110-114 -0.13024138279274666 1105 115-119 -0.07919318292028521 1105 120-124 -0.1852113948637779 1105 125-129 -0.07349134252970657 1105 130-134 -0.25612701663602166 1105 135-139 0.05077465996256336 1105 140-144 -0.016059022463494443 1105 145-149 -0.06692816578738103 1105 150-151 0.11159849418235268 1106 1 -0.22870611068034918 1106 2 -0.09726201048036032 1106 3 -0.11459188305379797 1106 4 -0.021747015018217297 1106 5 -0.08995097352890014 1106 6 -0.15826656699233155 1106 7 -0.032780862933051935 1106 8 -0.10861546547601364 1106 9 0.16403580422635855 1106 10-14 0.04339134790944854 1106 15-19 0.06851298487644186 1106 20-24 0.09588600833509275 1106 25-29 0.0972905204405663 1106 30-34 0.14359238505534933 1106 35-39 0.2136013679405835 1106 40-44 0.20972646490280056 1106 45-49 0.1378906651476015 1106 50-54 0.01528650244751617 1106 55-59 -0.06350099744435767 1106 60-64 -0.06510225876711928 1106 65-69 0.024558673683408472 1106 70-74 0.08499653534003926 1106 75-79 0.11494572882589438 1106 80-84 0.10247181198651845 1106 85-89 -0.0036767721029420386 1106 90-94 0.04646564962248334 1106 95-99 0.044037939764479006 1106 100-104 -0.03904001411380165 1106 105-109 0.023493957722038772 1106 110-114 0.15645611521139102 1106 115-119 0.08230127604795712 1106 120-124 0.05115911630591796 1106 125-129 0.14463764493523001 1106 130-134 0.21334958765571344 1106 135-139 0.1501891246783984 1106 140-144 0.09206099059244366 1106 145-149 0.06331856542814052 1106 150-151 0.14824446210860032 1107 1 -0.19764998573352344 1107 2 -0.3042974928756017 1107 3 -0.30126983533605767 1107 4 -0.1452401311837086 1107 5 -0.15065529966096847 1107 6 -0.12794285462571509 1107 7 -0.27250892569458074 1107 8 -0.15675147648833843 1107 9 -0.13166008341797664 1107 10-14 -0.024815895834116475 1107 15-19 0.02702832551415213 1107 20-24 0.1240208998406871 1107 25-29 0.010850298704148997 1107 30-34 -0.006628316229189579 1107 35-39 0.08177167207804814 1107 40-44 0.00320023459268981 1107 45-49 -0.13060536605699724 1107 50-54 -0.05457193139961447 1107 55-59 -0.053663887730841964 1107 60-64 -0.060504852112678975 1107 65-69 -0.09347561244481284 1107 70-74 -3.6956242476026304 1107 75-79 -0.18383442042657094 1107 80-84 0.0011305029909252085 1107 85-89 0.05561574080482501 1107 90-94 0.021571462558270582 1107 95-99 0.021501754899347247 1107 100-104 -0.02312706975555301 1107 105-109 0.011591378679000286 1107 110-114 5.756283165183618E-4 1107 115-119 0.01031849587235456 1107 120-124 -0.03171502376783053 1107 125-129 -0.05686284392289309 1107 130-134 0.06191343956555073 1107 135-139 -0.04434543937149016 1107 140-144 -0.030876446885471864 1107 145-149 -0.021231109904611856 1107 150-151 -0.054160141615859914 1108 1 -0.04845680915445172 1108 2 0.09543523642595275 1108 3 -0.10257781701568192 1108 4 -0.057949932257212566 1108 5 0.04805162414066899 1108 6 -0.13898432116059212 1108 7 0.005448797220751089 1108 8 -0.08348877457689241 1108 9 0.17507683432381782 1108 10-14 0.027576274573561932 1108 15-19 -0.057021421786544124 1108 20-24 -0.04491665287460478 1108 25-29 0.04668897616650014 1108 30-34 0.042503357250318174 1108 35-39 -0.13764965602089774 1108 40-44 -0.16933893469914807 1108 45-49 -0.005584418716011896 1108 50-54 -0.02456181109182154 1108 55-59 0.09403532788103774 1108 60-64 0.002192022310694597 1108 65-69 0.05445006368581318 1108 70-74 0.1452338763729344 1108 75-79 -0.013675481737955408 1108 80-84 0.07414593838774408 1108 85-89 0.10472372998857793 1108 90-94 0.02827666760371983 1108 95-99 0.045706241654805524 1108 100-104 0.1880813381694324 1108 105-109 0.146011516909347 1108 110-114 0.12638681287566556 1108 115-119 0.13132141888863202 1108 120-124 0.09302775645392813 1108 125-129 0.0954443241230436 1108 130-134 0.23850829779129157 1108 135-139 0.13357668514273513 1108 140-144 0.08389633447219325 1108 145-149 0.09923480460062706 1108 150-151 -0.12911672297691013 1109 1 -0.31394278849242596 1109 2 -0.02088660476182369 1109 3 -0.15346806870619645 1109 4 0.02309765274219089 1109 5 -0.225636832073981 1109 6 0.04996476322615706 1109 7 -0.011572167312799309 1109 8 -0.00982751328259468 1109 9 0.020028204306122177 1109 10-14 -0.066133020894668 1109 15-19 -0.048022958757883316 1109 20-24 -0.048530582785041076 1109 25-29 0.03329434731605119 1109 30-34 -0.10923134255137512 1109 35-39 -0.2547378439530803 1109 40-44 -0.290640807481914 1109 45-49 -0.1141026011175299 1109 50-54 -0.16332838395128846 1109 55-59 -0.1935434380806953 1109 60-64 -0.0870583451262874 1109 65-69 -0.3359551406274832 1109 70-74 -0.05790704525986001 1109 75-79 -0.09200467472106055 1109 80-84 -0.21272007621195144 1109 85-89 -0.06330005487210144 1109 90-94 -0.11334958863353961 1109 95-99 -0.1831595468298346 1109 100-104 -0.0640760745852873 1109 105-109 -0.15369123344433433 1109 110-114 -0.15749191611320157 1109 115-119 -0.07248972002852128 1109 120-124 -0.18766360564490014 1109 125-129 -0.2472362394534926 1109 130-134 -0.0653487363734726 1109 135-139 -0.11123797689163695 1109 140-144 -0.07252634181352846 1109 145-149 -0.06489191761701107 1109 150-151 -0.2932892487738954 1110 1 -0.28211826047210664 1110 2 -0.07660410569623721 1110 3 -0.1498294096768511 1110 4 -0.08745592322608431 1110 5 -0.07386170165420936 1110 6 0.033963039304168774 1110 7 -0.15972564591722715 1110 8 -0.0030188275312781343 1110 9 0.07124498690694026 1110 10-14 -0.019541748289071847 1110 15-19 -0.024596357076475783 1110 20-24 -0.10306853020502871 1110 25-29 -0.01688898651865145 1110 30-34 -0.06085944413143096 1110 35-39 -0.18496451964044525 1110 40-44 -0.22931379650666628 1110 45-49 -0.08813415824889859 1110 50-54 -0.22259937021438247 1110 55-59 -0.19933320492849305 1110 60-64 -0.14363241980112917 1110 65-69 -0.4210875717723539 1110 70-74 -0.12129829946226067 1110 75-79 -0.2576393672355408 1110 80-84 -0.3106316296548073 1110 85-89 -0.20998603701511342 1110 90-94 -0.16365047869085458 1110 95-99 -0.14438720814915484 1110 100-104 -0.11153438085946021 1110 105-109 -0.30719120994882587 1110 110-114 -0.25672346530130596 1110 115-119 -0.19929985286763952 1110 120-124 -0.29630283780753075 1110 125-129 -0.41065248344664695 1110 130-134 -0.12079750366503816 1110 135-139 -0.003731385858891656 1110 140-144 -0.004488028590984072 1110 145-149 -0.250867415038698 1110 150-151 -0.24433093571649778 1111 1 0.1527225825277334 1111 2 0.12682180881325067 1111 3 -0.0316784746322476 1111 4 0.13238013548359007 1111 5 -0.08935425392588314 1111 6 0.09618871925898276 1111 7 -0.21337558946859048 1111 8 0.07348726874843692 1111 9 0.05456837347044541 1111 10-14 0.014020737010255857 1111 15-19 0.00812400053644069 1111 20-24 -0.026302235688412168 1111 25-29 -0.028318355673476958 1111 30-34 0.05803768195636394 1111 35-39 -0.03816063578861417 1111 40-44 -0.05859216655620969 1111 45-49 -0.08600921562854325 1111 50-54 -0.033242529702107504 1111 55-59 -0.06560788589381161 1111 60-64 -0.02234940033614663 1111 65-69 -0.16249575266733984 1111 70-74 0.03188596932087506 1111 75-79 -0.11644916627992075 1111 80-84 0.030381960662147378 1111 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115-119 0.04590480086611848 2112 120-124 0.09477646301224496 2112 125-129 0.1558685404843203 2112 130-134 0.2062599333429489 2112 135-139 0.21188675549078795 2112 140-144 0.25259326740336263 2112 145-149 0.17118305806643264 2112 150-151 0.24858278082816554 2113 1 0.417653799494591 2113 2 0.10511610262200222 2113 3 0.3013753700259443 2113 4 0.28976313714517943 2113 5 0.2745434631372632 2113 6 0.3980941265180533 2113 7 0.16430137776396236 2113 8 0.23878184906348565 2113 9 0.05597788716389118 2113 10-14 0.11092751691708713 2113 15-19 0.14914492859603 2113 20-24 0.14420913595443352 2113 25-29 0.11795920511928415 2113 30-34 0.04596322849381096 2113 35-39 0.16536477653437487 2113 40-44 0.09558877630215079 2113 45-49 0.2383449802665396 2113 50-54 0.2091024092885263 2113 55-59 0.1732503219316044 2113 60-64 0.11783208858714289 2113 65-69 0.11453402097022547 2113 70-74 0.2802676578441705 2113 75-79 0.16313137427194846 2113 80-84 0.10073881352422376 2113 85-89 0.08878279816661205 2113 90-94 0.10377550077514996 2113 95-99 0.13941696040858176 2113 100-104 0.13470501156034942 2113 105-109 0.12765028115365595 2113 110-114 0.017724881489172617 2113 115-119 0.1174356200200748 2113 120-124 0.1280415850837855 2113 125-129 0.15006110577341758 2113 130-134 0.1606238232865067 2113 135-139 0.10809794559916952 2113 140-144 0.25020385970331205 2113 145-149 0.13794255955643564 2113 150-151 0.19774771470006414 2114 1 0.3717580707754955 2114 2 0.3893516576905789 2114 3 0.4119135210611944 2114 4 0.12518827533899923 2114 5 0.35504437410295964 2114 6 0.4001511900639585 2114 7 0.23078483083070012 2114 8 0.2594374720434942 2114 9 0.1921890319887467 2114 10-14 0.1270689134886851 2114 15-19 0.13773461368801776 2114 20-24 0.10662907976580271 2114 25-29 0.11428500267918906 2114 30-34 0.10917367505022213 2114 35-39 0.15898051395693358 2114 40-44 0.2957506181297731 2114 45-49 0.28476783266158634 2114 50-54 0.2087576889372258 2114 55-59 0.2236776583779161 2114 60-64 0.20822322583257602 2114 65-69 0.2036346455553968 2114 70-74 0.3763250136374907 2114 75-79 0.2205064862521482 2114 80-84 0.21631317883878864 2114 85-89 0.20977792344690016 2114 90-94 0.13508568941728782 2114 95-99 0.13894298591780796 2114 100-104 0.1635187785883545 2114 105-109 0.15419550278512872 2114 110-114 0.173436897344736 2114 115-119 0.17926864732218206 2114 120-124 0.18053358826066557 2114 125-129 0.06942127219543437 2114 130-134 0.24081302195745025 2114 135-139 0.29152689598591763 2114 140-144 0.2719541219537831 2114 145-149 0.25683425283043704 2114 150-151 0.42270374279639356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 19.0 15 77.0 16 173.0 17 282.0 18 537.0 19 992.0 20 1384.0 21 1638.0 22 1908.0 23 2321.0 24 2803.0 25 3233.0 26 4195.0 27 5215.0 28 6298.0 29 7664.0 30 9199.0 31 11289.0 32 13872.0 33 17652.0 34 24363.0 35 36328.0 36 67229.0 37 289782.0 38 377494.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.57638692928495 17.05942773294204 8.921270952085331 24.44291438568768 2 21.17241379310345 17.994582087025226 33.41497827191151 27.418025847959814 3 23.373892431852813 22.849483605169592 28.739206501495566 25.037417461482026 4 13.40820588069304 13.339240363451662 38.294147525255376 34.95840623059992 5 14.126869462159263 36.205542073480444 35.692646311868614 13.974942152491677 6 33.58756137479541 37.42637846379592 15.412720808172017 13.573339353236639 7 25.70619109430555 28.77950222924544 22.259834076415146 23.254472600033864 8 27.05841187425927 32.35159997742536 18.22123144646989 22.368756701845477 9 23.820983125458547 10.770923867035386 18.21863536316948 47.189457644336585 10-14 26.50406907839043 20.33505276821491 26.594909419267452 26.56596873412721 15-19 25.842541904170663 26.150324510412553 23.489610023139004 24.51752356227778 20-24 25.398408488063662 24.65227157288786 24.55867712624866 25.39064281279982 25-29 25.303030645070262 24.603013714092217 24.845803939274226 25.248151701563295 30-34 25.45642530616852 24.58344150347085 24.596060725774592 25.364072464586034 35-39 25.330052486031946 24.66556803431345 24.794333766013885 25.21004571364072 40-44 25.341633275015518 24.604345617698517 24.695185958575543 25.358835148710423 45-49 25.27806309611152 24.55411704949489 24.822236017833962 25.345583836559626 50-54 25.34282973079745 24.627484621028277 24.72755798859981 25.30212765957447 55-59 25.29034369885434 24.47086178678255 24.76785371634968 25.47094079801343 60-64 25.24564591681246 24.607167447372877 24.832778373497373 25.314408262317283 65-69 25.391049156272928 24.550347085049946 24.629651786218183 25.42895197245894 70-74 25.2220779953722 24.394988430498334 25.129905750888877 25.253027823240586 75-79 25.358135334951182 24.531497262825216 24.694147525255374 25.416219876968228 80-84 25.30759072182403 24.672814492917208 24.729973474801064 25.2896213104577 85-89 25.37170269202551 24.52648569332355 24.649065974377788 25.452745640273157 90-94 25.301811614650937 24.537502116372256 24.789119024775662 25.371567244201138 95-99 25.345628985834416 24.576059597042725 24.700626446187705 25.377684970935157 100-104 25.32203848975676 24.522580281054235 24.79632033410463 25.359060895084372 105-109 25.30106665161691 24.640555336079913 24.746385236187145 25.311992776116032 110-114 25.394661098256112 24.55960268638185 24.695614876686044 25.350121338675997 115-119 25.272690332411536 24.601072295276257 24.843072408149443 25.283164964162765 120-124 25.26009368474519 24.550572831423896 24.76826005982279 25.421073424008128 125-129 25.253501890625884 24.706924770020883 24.771894576443366 25.267678762909874 130-134 25.256730063773354 24.51804277893786 24.800993284045376 25.424233873243413 135-139 25.219007844686498 24.647124555561824 24.832033410463346 25.301834189288336 140-144 25.24869349286077 24.596895987358202 24.78449122410971 25.369919295671313 145-149 25.20853321293527 24.59064281279982 24.846977820418758 25.353846153846156 150-151 25.18799029290592 24.636322591568373 24.883797054009822 25.291890061515886 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 1.5 3 1.0 4 1.0 5 1.0 6 1.0 7 1.0 8 0.5 9 1.0 10 1.0 11 3.0 12 2.5 13 3.0 14 7.5 15 11.0 16 25.0 17 43.0 18 58.5 19 83.5 20 125.0 21 197.0 22 269.5 23 385.0 24 557.5 25 729.5 26 972.0 27 1265.5 28 1587.5 29 1960.0 30 2408.5 31 2890.0 32 3406.5 33 3977.0 34 4547.5 35 5343.5 36 6292.5 37 7096.5 38 8121.5 39 9457.0 40 10924.5 41 12718.0 42 15021.0 43 17648.0 44 20593.5 45 24221.0 46 28207.5 47 32318.0 48 37168.5 49 42338.5 50 47290.0 51 51781.0 52 54915.0 53 56519.5 54 56564.5 55 54377.0 56 50350.5 57 44988.5 58 38708.0 59 32411.5 60 26016.0 61 20284.5 62 15233.0 63 10945.0 64 7816.0 65 5407.5 66 3611.5 67 2277.0 68 1388.0 69 809.0 70 464.0 71 272.5 72 188.5 73 124.5 74 64.0 75 40.0 76 29.5 77 16.0 78 9.0 79 6.5 80 8.5 81 9.5 82 5.5 83 2.0 84 1.5 85 2.0 86 1.5 87 1.0 88 1.0 89 0.5 90 1.5 91 1.5 92 1.0 93 0.5 94 0.0 95 1.0 96 1.0 97 0.5 98 0.5 99 1.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 885950.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.9681374620546 #Duplication Level Percentage of deduplicated Percentage of total 1 78.88168673274897 59.13613134219453 2 14.182520033211404 21.264742228162714 3 4.22438630994346 9.500831207299889 4 1.5032677162355261 4.507887231720551 5 0.6443582210124794 2.415316784383425 6 0.26202492955899165 1.1786112562598194 7 0.1377289697351072 0.7227699034926072 8 0.06568435535502072 0.3939387025089308 9 0.03463549938147021 0.23369029908272687 >10 0.06370723281751839 0.6460810448948089 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1287318697443423E-4 0.0 10-14 0.0 0.0 0.0 1.5802246176420794E-4 0.0 15-19 0.0 0.0 0.0 2.93470286133529E-4 0.0 20-24 0.0 0.0 0.0 3.3861956092330267E-4 0.0 25-29 0.0 0.0 0.0 0.0029572774987301764 0.0 30-34 0.0 0.0 0.0 0.026570348213781812 0.0 35-39 0.0 0.0 0.0 0.08025283593882274 0.0 40-44 0.0 0.0 0.0 0.12522151362943731 0.0 45-49 0.0 0.0 0.0 0.21396241322873752 0.0 50-54 0.0 0.0 0.0 0.26879620746091765 0.0 55-59 0.0 0.0 0.0 0.36187143744003614 0.0 60-64 0.0 0.0 0.0 0.42742818443478753 0.0 65-69 0.0 0.0 0.0 0.5286302838760653 0.0 70-74 0.0 0.0 0.0 0.6003950561544105 0.0 75-79 0.0 0.0 0.0 0.7212822394040296 0.0 80-84 0.0 0.0 0.0 0.8102940346520684 0.0 85-89 0.0 0.0 0.0 0.9438681641176139 0.0 90-94 0.0 0.0 0.0 1.0514137366668548 0.0 95-99 0.0 0.0 0.0 1.2061628760088041 0.0 100-104 0.0 0.0 0.0 1.335086630171003 0.0 105-109 0.0 0.0 0.0 1.5081212258028107 0.0 110-114 0.0 0.0 0.0 1.6678142107342402 0.0 115-119 0.0 0.0 0.0 1.869766916868898 0.0 120-124 0.0 0.0 0.0 2.0580168181048593 0.0 125-129 0.0 0.0 0.0 2.2837631920537276 0.0 130-134 0.0 0.0 0.0 2.5142276652181272 0.0 135-139 0.0 0.0 0.0 2.7654608047858233 0.0 140 0.0 0.0 0.0 2.945877306845759 0.0 >>END_MODULE