FastQCFastQC Report
Fri 3 Apr 2020
Trimmomatic on Trim Galore_ on data 9 and data 8_ trimmed reads pair 1 _R1 unpaired_

Summary

[OK]Basic Statistics

MeasureValue
FilenameTrimmomatic on Trim Galore_ on data 9 and data 8_ trimmed reads pair 1 _R1 unpaired_
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6651
Sequences flagged as poor quality0
Sequence length15-36
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGACCGAT741.1126146444143736Illumina Paired End PCR Primer 2 (100% over 27bp)
CGGTTCAGCAGGAATGCCGAGACCGATA370.5563073222071868Illumina Paired End PCR Primer 2 (96% over 27bp)
CGGTTCAGCAGGAATGCCGAGACCGA180.2706359945872801Illumina Paired End PCR Primer 2 (100% over 26bp)
CGGTTCAGCAGGAATGCCGAGACCG140.21049466245677342Illumina Paired End PCR Primer 2 (100% over 25bp)
CGGTTCAGCAGGAATGCCGAGACCGATAT70.10524733122838671Illumina Paired End PCR Primer 2 (96% over 29bp)
CAGCAGGAATGCCGAGACCGAT70.10524733122838671Illumina Paired End PCR Primer 2 (100% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)