FastQCFastQC Report
Sat 3 Jul 2021
Trimmomatic on G1E_rep2_forward_read_X28SRR549356_1X29 _R1 paired_

Summary

[OK]Basic Statistics

MeasureValue
FilenameTrimmomatic on G1E_rep2_forward_read_X28SRR549356_1X29 _R1 paired_
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616265
Sequences flagged as poor quality0
Sequence length1-99
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACG183241.1337249770303754No Hit
TGGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGAC129340.8002400596436846No Hit
CTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGG113650.7031643944526423No Hit
GGGGAATACAGCCCGGGGAGCATCGTCGCCCGCGAAGCCGGCTTTGCACA106590.6594834386687827No Hit
GGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACAGAAGCAATGC94670.5857331563821527No Hit
CCCATGTTTAGTTGATTTTCCTCCGCGAGGCACAGAGTCGCCCAGTGCCC74220.45920687511020775No Hit
GGGAAGCCGAAGAGATGGGAGCCCGAGTGGGCTGCAGTGGGGGTGAGGGA72960.4514111237946748No Hit
GCCAGATCTTCTCCATGTCGTCCCAGTTGGTAACAATGCCATGTTCAATG56640.35043758294586597No Hit
GTTGAAGGTCTCAAACATGATCTGGGTCATCTTTTCACGGTTGGCCTTAG47940.2966097762433759No Hit
GTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCT47900.2963622920746289No Hit
GGGGTGTTGAAGGTCTCAAACATGATCTGGGTCATCTTTTCACGGTTGGC46810.28961834847627094No Hit
GCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGATC44040.2724800697905356No Hit
GGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTG43340.2681490968374617No Hit
TGGGTATCGGGTGGTGTGTTGCAGGCTGGGTGCCCATGGTAGCTAGGCCC42520.2630756713781465No Hit
GGGAATACAGCCCGGGGAGCATCGTCGCCCGCGAAGCCGGCTTTGCACAT41960.2596108930156874No Hit
CGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACAGAAGCAATG40630.25138204440484696No Hit
CTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAA36380.2250868514754697No Hit
CCGGGGAGCATCGTCGCCCGCGAAGCCGGCTTTGCACATGCCGGAGCCGT35250.21809542370836466No Hit
GTTGCAGGCTGGGTGCCCATGGTAGCTAGGCCCGGGCGCAGGGGACTGCC35110.2172292291177499No Hit
CCTCTCAGCTGTGGTGGTGAAGCTGTAGCCACGCTCGGTCAGGATCTTCA32610.20176146857105734No Hit
GCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGG32080.1984823033351585No Hit
GGAATACAGCCCGGGGAGCATCGTCGCCCGCGAAGCCGGCTTTGCACATG30500.18870667866964885No Hit
GGGGGGTTCAGTGAAGCCAGGAAGACCTTGTGCTGTTCCTTGACACGTTC29630.18332389799939985No Hit
GTTTAGTTGATTTTCCTCCGCGAGGCACAGAGTCGCCCAGTGCCCGTCCG28280.17497130730418586No Hit
CGGCGTAGTTGTGCTGGTGAGTGGAGACGTGGCACCTCTTGAGGACCAGT26940.1666805876511587No Hit
TCCCTCTCAGCTGTGGTGGTGAAGCTGTAGCCACGCTCGGTCAGGATCTT26490.16389639075275403No Hit
GG26290.16265896990901862No Hit
GGGCGGTGTCTCCTCATGCAGCACTAGGGGCTCAGGGCTGGCCCGTGGGG25670.1588229652934389No Hit
TTGAAGTTGACGGGATCCACACGCAGCTTGTGGGCATGCAGGTCGCTCAG25440.15739993132314317No Hit
GTGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGT25440.15739993132314317No Hit
CTCAGCTGTGGTGGTGAAGCTGTAGCCACGCTCGGTCAGGATCTTCATGA24910.15412076608724434No Hit
CTTGAAGTTGACGGGATCCACACGCAGCTTGTGGGCATGCAGGTCGCTCA24790.15337831358100312No Hit
GGGATGGGCAAACGGGGGAGCCAGGGTCAGCTGGAGGGCTGGGCTCAGCT24000.14849050124824828No Hit
CTGGGGTGTTGAAGGTCTCAAACATGATCTGGGTCATCTTTTCACGGTTG23990.1484286302060615No Hit
GTTCAGGGGGGCCTCGGTGAGCAGCACAGGGTGCTCCTCAGGGGCCACAC23270.14397391516861408No Hit
CCTCTCATTTGGTTCGCTGCTTCCTCAGAAAGATTCCCATGAACGACGGA23170.14335520474674637No Hit
GTCTTGGAGAACGGGCTGACGGTCCAGGGACTGTGGTGATGGGCAGCGGC23140.14316959162018605No Hit
TGAAGCTGTAGCCACGCTCGGTCAGGATCTTCATGAGGTAGTCTGTCAGG23080.14279836536706544No Hit
CCACGATGGAGGGGAATACAGCCCGGGGAGCATCGTCGCCCGCGAAGCCG22900.14168468660770356No Hit
GTGAAGCTGTAGCCACGCTCGGTCAGGATCTTCATGAGGTAGTCTGTCAG21960.13586880864214718No Hit
GTCAGGATCTTCATGAGGTAGTCTGTCAGGTCCCGGCCAGCCAGGTCCAG21560.13339396695467637No Hit
GCGGTGTCTCCTCATGCAGCACTAGGGGCTCAGGGCTGGCCCGTGGGGAG20860.12906299400160245No Hit
CTCAGGAGCTTGAAGTTGACGGGATCCACACGCAGCTTGTGGGCATGCAG20850.12900112295941568No Hit
GGTAGGAGCAGTGCCAGAAGCAGCAACCCCAGCCCTAGCCTGGCCACCAT20660.12782557315786705No Hit
CGGGTGGTGTGTTGCAGGCTGGGTGCCCATGGTAGCTAGGCCCGGGCGCA20630.12763996003130673No Hit
TTGGAGAACGGGCTGACGGTCCAGGGACTGTGGTGATGGGCAGCGGCGGC20460.12658815231413165No Hit
TGAAGGTCTCAAACATGATCTGGGTCATCTTTTCACGGTTGGCCTTAGGG20210.1250413762594624No Hit
GCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACAGAAGCAAT19660.12163846893919003No Hit
CCATAATCATCAGCTCCATCTTGGTCTTCATGTCCTCCGGCCTCCTCCGC19230.11897801412515893No Hit
CCATGTTTAGTTGATTTTCCTCCGCGAGGCACAGAGTCGCCCAGTGCCCG19200.11879240099859861No Hit
TGTCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGAC19010.11761685119704998No Hit
GTTCAGTGAAGCCAGGAAGACCTTGTGCTGTTCCTTGACACGTTCCACCA18730.11588446201582044No Hit
GGAAGCCGAAGAGATGGGAGCCCGAGTGGGCTGCAGTGGGGGTGAGGGAG18470.1142758149189644No Hit
TGAGGAAACGCTTAAGCTTGCTGAGCTGCCGTGCCCCGCACGCCGGGGGC17940.11099664968306558No Hit
TGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTT16740.10357212462065318No Hit
TTCAGGGGGGCCTCGGTGAGCAGCACAGGGTGCTCCTCAGGGGCCACACG16430.10165412231286329No Hit
TCTCATTTGGTTCGCTGCTTCCTCAGAAAGATTCCCATGAACGACGGAAA16260.10060231459568819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)