##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	A3_right_fq_gz.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10000
Total Bases	1 Mbp
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9882	34.0	33.0	34.0	31.0	34.0
2	33.1593	34.0	33.0	34.0	31.0	34.0
3	33.1522	34.0	34.0	34.0	31.0	34.0
4	36.3928	37.0	37.0	37.0	35.0	37.0
5	36.4488	37.0	37.0	37.0	35.0	37.0
6	36.3758	37.0	37.0	37.0	35.0	37.0
7	36.413	37.0	37.0	37.0	35.0	37.0
8	36.487	37.0	37.0	37.0	35.0	37.0
9	38.3212	39.0	39.0	39.0	37.0	39.0
10-11	38.270399999999995	39.0	39.0	39.0	37.0	39.0
12-13	38.18875	39.0	39.0	39.0	37.0	39.0
14-15	39.82425	41.0	40.0	41.0	38.0	41.0
16-17	39.744150000000005	41.0	40.0	41.0	38.0	41.0
18-19	39.66385	41.0	40.0	41.0	38.0	41.0
20-21	39.56235	41.0	40.0	41.0	37.5	41.0
22-23	39.42325	41.0	40.0	41.0	37.0	41.0
24-25	39.3334	41.0	40.0	41.0	37.0	41.0
26-27	39.4187	41.0	40.0	41.0	37.0	41.0
28-29	39.211749999999995	41.0	40.0	41.0	36.0	41.0
30-31	39.0338	41.0	39.0	41.0	36.0	41.0
32-33	38.7948	41.0	39.0	41.0	35.0	41.0
34-35	38.84415	41.0	39.0	41.0	35.0	41.0
36-37	38.78535	41.0	39.0	41.0	35.0	41.0
38-39	38.5202	40.0	38.5	41.0	35.0	41.0
40-41	38.27885	40.0	38.0	41.0	34.5	41.0
42-43	38.2072	40.0	38.0	41.0	34.0	41.0
44-45	38.02945	40.0	38.0	41.0	33.5	41.0
46-47	37.7227	40.0	37.5	41.0	33.0	41.0
48-49	37.57925	40.0	37.0	41.0	33.0	41.0
50-51	36.7672	39.0	36.0	40.0	32.0	40.5
52-53	37.0481	39.5	36.5	40.5	32.5	40.5
54-55	37.464200000000005	40.0	36.0	41.0	33.0	41.0
56-57	37.26835	40.0	36.0	41.0	33.0	41.0
58-59	37.079750000000004	40.0	35.0	41.0	32.5	41.0
60-61	36.7992	39.0	35.0	41.0	32.0	41.0
62-63	36.47725	39.0	35.0	41.0	31.5	41.0
64-65	36.1221	38.5	35.0	41.0	31.0	41.0
66-67	35.781099999999995	37.5	35.0	40.5	30.5	41.0
68-69	35.339749999999995	37.0	35.0	40.0	30.0	41.0
70-71	34.89295	36.0	34.5	39.0	29.0	41.0
72-73	34.515299999999996	36.0	34.0	39.0	29.0	41.0
74-75	34.024	35.0	34.0	38.5	28.5	40.0
76-77	33.61325	35.0	34.0	37.0	28.0	39.0
78-79	33.1251	35.0	33.5	37.0	27.0	39.0
80-81	32.5814	35.0	33.0	36.5	25.5	38.5
82-83	32.21495	35.0	33.0	36.0	25.0	37.0
84-85	31.8366	35.0	33.0	35.5	24.5	37.0
86-87	31.45265	35.0	32.0	35.0	23.0	36.5
88-89	31.17925	35.0	32.0	35.0	21.5	36.0
90-91	30.86775	35.0	32.0	35.0	20.0	36.0
92-93	30.46075	34.0	31.0	35.0	18.5	36.0
94-95	30.1308	34.0	31.0	35.0	12.5	35.0
96-97	29.6774	34.0	30.5	35.0	2.0	35.0
98-99	29.134549999999997	34.0	30.0	35.0	2.0	35.0
100-101	26.666	32.0	25.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-11	0.0
1101	12-13	0.0
1101	14-15	0.0
1101	16-17	0.0
1101	18-19	0.0
1101	20-21	0.0
1101	22-23	0.0
1101	24-25	0.0
1101	26-27	0.0
1101	28-29	0.0
1101	30-31	0.0
1101	32-33	0.0
1101	34-35	0.0
1101	36-37	0.0
1101	38-39	0.0
1101	40-41	0.0
1101	42-43	0.0
1101	44-45	0.0
1101	46-47	0.0
1101	48-49	0.0
1101	50-51	0.0
1101	52-53	0.0
1101	54-55	0.0
1101	56-57	0.0
1101	58-59	0.0
1101	60-61	0.0
1101	62-63	0.0
1101	64-65	0.0
1101	66-67	0.0
1101	68-69	0.0
1101	70-71	0.0
1101	72-73	0.0
1101	74-75	0.0
1101	76-77	0.0
1101	78-79	0.0
1101	80-81	0.0
1101	82-83	0.0
1101	84-85	0.0
1101	86-87	0.0
1101	88-89	0.0
1101	90-91	0.0
1101	92-93	0.0
1101	94-95	0.0
1101	96-97	0.0
1101	98-99	0.0
1101	100-101	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12.0
3	0.0
4	9.0
5	14.0
6	4.0
7	11.0
8	12.0
9	17.0
10	6.0
11	13.0
12	17.0
13	14.0
14	13.0
15	19.0
16	17.0
17	27.0
18	20.0
19	28.0
20	37.0
21	23.0
22	31.0
23	49.0
24	50.0
25	65.0
26	65.0
27	79.0
28	82.0
29	115.0
30	127.0
31	183.0
32	218.0
33	305.0
34	502.0
35	737.0
36	1203.0
37	2042.0
38	2993.0
39	840.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.060000000000002	12.2	11.690000000000001	52.05
2	21.418553396113	20.90763374073332	38.29893808855941	19.374874774594268
3	25.78610054075706	23.583016222711798	24.494292008812337	26.13659122771881
4	26.36955433149725	31.25688532799199	18.207310966449676	24.166249374061092
5	27.41289547456948	30.997196635963153	21.245494593512216	20.344413295955146
6	21.87	33.879999999999995	21.69	22.56
7	19.439999999999998	16.28	40.0	24.279999999999998
8	20.93	21.83	28.63	28.610000000000003
9	20.89	21.26	31.77	26.08
10-11	25.374999999999996	30.075000000000003	21.16	23.39
12-13	22.81	23.565	27.944999999999997	25.679999999999996
14-15	23.91	25.259999999999998	26.755000000000003	24.075
16-17	24.39	25.779999999999998	25.25	24.58
18-19	24.240000000000002	25.82	25.22	24.72
20-21	24.825	25.814999999999998	25.255	24.104999999999997
22-23	24.195	26.015	25.35	24.44
24-25	24.04	26.169999999999998	25.655	24.135
26-27	23.7	26.195	25.705	24.4
28-29	24.135	25.759999999999998	25.255	24.85
30-31	24.305	25.215	25.919999999999998	24.560000000000002
32-33	24.560000000000002	26.009999999999998	25.745	23.685000000000002
34-35	24.825	25.385	25.435000000000002	24.355
36-37	24.104999999999997	26.009999999999998	25.415	24.47
38-39	24.765	26.435	25.09	23.71
40-41	24.235	25.27	25.995	24.5
42-43	24.285	25.605	25.755	24.355
44-45	23.745	25.795	25.89	24.57
46-47	24.01	25.759999999999998	25.805	24.425
48-49	24.16	25.979999999999997	25.75	24.11
50-51	24.52	26.035000000000004	25.224999999999998	24.22
52-53	24.34	25.480000000000004	25.369999999999997	24.81
54-55	24.05	25.81	25.82	24.32
56-57	23.775	26.255	25.480000000000004	24.490000000000002
58-59	24.26	26.005	25.180000000000003	24.555
60-61	24.625	25.674999999999997	25.21	24.490000000000002
62-63	24.075	26.075	25.255	24.595
64-65	25.27	25.395	24.795	24.54
66-67	24.365000000000002	25.94	25.47	24.224999999999998
68-69	24.884999999999998	25.52	25.564999999999998	24.03
70-71	24.185000000000002	25.205	25.955000000000002	24.654999999999998
72-73	24.46	25.64	25.635	24.265
74-75	24.395	25.44	25.22	24.945
76-77	24.44	25.655	25.865	24.04
78-79	24.865000000000002	25.61	25.135	24.39
80-81	24.785	25.724999999999998	25.480000000000004	24.01
82-83	24.47	25.6	25.39	24.54
84-85	24.88	25.185000000000002	25.564999999999998	24.37
86-87	24.455	25.064999999999998	25.779999999999998	24.7
88-89	24.545	25.430000000000003	25.629999999999995	24.395
90-91	24.88	25.495	24.89	24.735
92-93	24.245	25.314999999999998	26.135	24.305
94-95	24.6	24.990000000000002	25.52	24.89
96-97	24.32	26.36	25.240000000000002	24.08
98-99	24.585	25.869999999999997	25.369999999999997	24.175
100-101	25.540000000000003	25.174999999999997	24.95	24.335
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	0.5
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	1.0
19	2.5
20	3.5
21	4.5
22	6.0
23	9.0
24	12.0
25	19.0
26	25.5
27	29.5
28	47.5
29	63.5
30	68.5
31	86.0
32	108.0
33	127.0
34	140.5
35	170.0
36	197.0
37	196.0
38	219.0
39	254.5
40	255.5
41	277.0
42	349.0
43	402.0
44	414.0
45	412.5
46	420.0
47	418.0
48	406.5
49	371.0
50	343.5
51	361.5
52	349.5
53	305.0
54	266.0
55	251.0
56	243.0
57	219.5
58	194.5
59	161.5
60	134.5
61	126.0
62	132.5
63	132.0
64	125.0
65	123.5
66	128.5
67	125.5
68	116.5
69	117.0
70	94.0
71	69.0
72	56.5
73	48.5
74	48.0
75	39.0
76	31.0
77	27.5
78	20.5
79	16.5
80	11.5
81	9.5
82	11.0
83	10.5
84	8.5
85	5.0
86	4.5
87	3.5
88	2.0
89	1.0
90	0.5
91	0.5
92	1.5
93	2.0
94	1.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.18
3	0.13999999999999999
4	0.15
5	0.12
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	10000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.48
#Duplication Level	Percentage of total
1	92.25
2	5.06
3	1.17
4	0.6799999999999999
5	0.4
6	0.06
7	0.21
8	0.08
9	0.09
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.04	0.0
2	0.0	0.0	0.0	0.0	0.04	0.0
3	0.0	0.0	0.0	0.0	0.04	0.0
4	0.0	0.0	0.0	0.0	0.04	0.0
5	0.0	0.0	0.0	0.0	0.04	0.0
6	0.0	0.0	0.0	0.0	0.04	0.0
7	0.0	0.0	0.0	0.0	0.04	0.0
8	0.0	0.0	0.0	0.0	0.05	0.0
9	0.0	0.0	0.0	0.0	0.06	0.0
10-11	0.0	0.0	0.0	0.0	0.06	0.0
12-13	0.0	0.0	0.0	0.0	0.07	0.0
14-15	0.0	0.0	0.0	0.0	0.08499999999999999	0.0
16-17	0.0	0.0	0.0	0.0	0.1	0.0
18-19	0.0	0.0	0.0	0.0	0.12	0.0
20-21	0.0	0.0	0.0	0.0	0.135	0.0
22-23	0.01	0.0	0.0	0.0	0.15	0.0
24-25	0.01	0.0	0.0	0.0	0.16	0.01
26-27	0.01	0.0	0.0	0.0	0.16	0.01
28-29	0.01	0.0	0.0	0.0	0.17	0.015
30-31	0.01	0.0	0.0	0.0	0.18	0.02
32-33	0.01	0.0	0.0	0.0	0.18	0.02
34-35	0.01	0.0	0.0	0.0	0.21	0.035
36-37	0.01	0.0	0.0	0.0	0.21	0.05
38-39	0.01	0.0	0.0	0.0	0.215	0.05
40-41	0.01	0.0	0.0	0.0	0.22	0.065
42-43	0.01	0.0	0.0	0.0	0.24	0.07
44-45	0.01	0.0	0.0	0.0	0.25	0.09
46-47	0.01	0.0	0.0	0.0	0.25	0.09
48-49	0.01	0.0	0.0	0.0	0.255	0.09
50-51	0.01	0.0	0.0	0.0	0.26	0.1
52-53	0.01	0.0	0.0	0.0	0.265	0.1
54-55	0.01	0.0	0.0	0.0	0.28	0.1
56-57	0.01	0.0	0.0	0.0	0.3	0.1
58-59	0.01	0.0	0.0	0.0	0.31	0.11
60-61	0.01	0.0	0.0	0.0	0.32	0.13
62-63	0.01	0.0	0.0	0.0	0.33	0.14500000000000002
64-65	0.01	0.0	0.0	0.0	0.33999999999999997	0.15
66-67	0.01	0.0	0.0	0.0	0.36	0.17
68-69	0.01	0.0	0.0	0.0	0.36	0.17
70-71	0.01	0.0	0.0	0.0	0.36	0.17
72-73	0.01	0.0	0.0	0.0	0.36	0.18
74-75	0.01	0.0	0.0	0.0	0.365	0.185
76-77	0.02	0.0	0.0	0.0	0.385	0.2
78-79	0.02	0.0	0.0	0.0	0.39	0.21
80-81	0.02	0.0	0.0	0.0	0.39	0.21
82-83	0.02	0.0	0.0	0.0	0.4	0.225
84-85	0.02	0.0	0.0	0.0	0.42	0.23
86-87	0.02	0.0	0.0	0.0	0.42	0.265
88-89	0.02	0.0	0.0	0.0	0.42	0.295
90	0.02	0.0	0.0	0.0	0.44	0.3
>>END_MODULE