##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	A1_left_fq_gz.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10000
Total Bases	1 Mbp
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8798	34.0	31.0	34.0	31.0	34.0
2	32.9296	34.0	33.0	34.0	31.0	34.0
3	33.0536	34.0	33.0	34.0	31.0	34.0
4	36.4594	37.0	37.0	37.0	35.0	37.0
5	36.3884	37.0	37.0	37.0	35.0	37.0
6	36.3379	37.0	37.0	37.0	35.0	37.0
7	36.3663	37.0	37.0	37.0	35.0	37.0
8	36.2895	37.0	37.0	37.0	35.0	37.0
9	38.1358	39.0	39.0	39.0	37.0	39.0
10-11	38.1194	39.0	39.0	39.0	37.0	39.0
12-13	38.045950000000005	39.0	38.5	39.0	35.0	39.0
14-15	39.567750000000004	41.0	40.0	41.0	36.5	41.0
16-17	39.5634	41.0	40.0	41.0	37.0	41.0
18-19	39.5389	41.0	39.5	41.0	37.0	41.0
20-21	39.31715	41.0	39.0	41.0	36.0	41.0
22-23	39.344899999999996	41.0	39.0	41.0	36.0	41.0
24-25	39.2448	41.0	39.0	41.0	36.0	41.0
26-27	39.07485	41.0	39.0	41.0	35.5	41.0
28-29	38.85435	40.5	39.0	41.0	35.0	41.0
30-31	38.5847	40.0	38.0	41.0	34.5	41.0
32-33	38.383300000000006	40.0	38.0	41.0	34.0	41.0
34-35	38.35105	40.0	38.0	41.0	34.0	41.0
36-37	38.1601	40.0	38.0	41.0	33.0	41.0
38-39	37.964	40.0	38.0	41.0	33.0	41.0
40-41	37.634100000000004	40.0	37.0	41.0	32.0	41.0
42-43	37.53125	40.0	37.0	41.0	32.0	41.0
44-45	37.2863	40.0	36.5	41.0	31.5	41.0
46-47	36.91905	39.5	36.0	41.0	30.5	41.0
48-49	36.8743	40.0	36.0	41.0	31.0	41.0
50-51	36.703	40.0	36.0	41.0	31.0	41.0
52-53	36.67585	39.5	35.0	41.0	30.5	41.0
54-55	36.6743	39.0	35.0	41.0	30.5	41.0
56-57	36.61935	39.0	35.0	41.0	31.0	41.0
58-59	36.472899999999996	39.0	35.0	41.0	31.0	41.0
60-61	36.217349999999996	39.0	35.0	41.0	31.0	41.0
62-63	35.85165	38.0	35.0	41.0	29.5	41.0
64-65	35.373400000000004	37.0	34.5	40.0	29.0	41.0
66-67	34.8964	37.0	34.0	40.0	28.0	41.0
68-69	34.6374	36.0	34.0	39.5	28.0	41.0
70-71	34.215599999999995	36.0	34.0	39.0	27.0	41.0
72-73	33.893950000000004	35.0	33.5	39.0	26.5	40.5
74-75	33.498850000000004	35.0	33.0	37.5	26.0	40.0
76-77	32.3094	34.5	31.5	36.5	25.0	39.0
78-79	32.8989	35.0	33.0	37.0	26.0	39.0
80-81	32.54325	35.0	33.0	36.0	25.5	38.5
82-83	32.1053	35.0	33.0	36.0	24.5	37.0
84-85	31.892400000000002	35.0	32.5	35.5	24.5	37.0
86-87	31.667050000000003	35.0	32.5	35.0	24.0	36.5
88-89	31.45975	35.0	33.0	35.0	23.5	36.0
90-91	31.15775	35.0	32.0	35.0	21.5	36.0
92-93	30.786099999999998	35.0	32.0	35.0	20.0	36.0
94-95	30.61995	35.0	32.0	35.0	18.5	35.0
96-97	30.285	35.0	31.0	35.0	14.0	35.0
98-99	29.9541	34.5	31.0	35.0	2.0	35.0
100-101	29.1956	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-11	0.0
1101	12-13	0.0
1101	14-15	0.0
1101	16-17	0.0
1101	18-19	0.0
1101	20-21	0.0
1101	22-23	0.0
1101	24-25	0.0
1101	26-27	0.0
1101	28-29	0.0
1101	30-31	0.0
1101	32-33	0.0
1101	34-35	0.0
1101	36-37	0.0
1101	38-39	0.0
1101	40-41	0.0
1101	42-43	0.0
1101	44-45	0.0
1101	46-47	0.0
1101	48-49	0.0
1101	50-51	0.0
1101	52-53	0.0
1101	54-55	0.0
1101	56-57	0.0
1101	58-59	0.0
1101	60-61	0.0
1101	62-63	0.0
1101	64-65	0.0
1101	66-67	0.0
1101	68-69	0.0
1101	70-71	0.0
1101	72-73	0.0
1101	74-75	0.0
1101	76-77	0.0
1101	78-79	0.0
1101	80-81	0.0
1101	82-83	0.0
1101	84-85	0.0
1101	86-87	0.0
1101	88-89	0.0
1101	90-91	0.0
1101	92-93	0.0
1101	94-95	0.0
1101	96-97	0.0
1101	98-99	0.0
1101	100-101	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	5.0
9	5.0
10	18.0
11	29.0
12	16.0
13	21.0
14	22.0
15	28.0
16	29.0
17	35.0
18	22.0
19	25.0
20	26.0
21	33.0
22	46.0
23	48.0
24	64.0
25	57.0
26	78.0
27	78.0
28	133.0
29	149.0
30	169.0
31	240.0
32	283.0
33	366.0
34	532.0
35	746.0
36	1221.0
37	1987.0
38	2659.0
39	826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.27	13.569999999999999	12.959999999999999	49.2
2	20.801124385101897	21.142455576749324	38.0684670213834	19.987953016765385
3	24.779999999999998	24.63	25.130000000000003	25.46
4	24.88	31.16	19.96	24.0
5	27.58	31.990000000000002	20.41	20.02
6	20.75	33.33	22.29	23.630000000000003
7	19.259999999999998	15.73	39.72	25.290000000000003
8	21.48	21.3	27.61	29.609999999999996
9	20.22011005502751	21.180590295147574	31.355677838919462	27.243621810905456
10-11	25.665	29.099999999999998	20.849999999999998	24.385
12-13	23.465	22.62	27.900000000000002	26.015
14-15	24.169999999999998	24.535	26.855	24.44
16-17	25.779999999999998	24.95	24.51	24.759999999999998
18-19	24.355	25.335	25.929999999999996	24.38
20-21	25.490000000000002	24.875	25.624999999999996	24.01
22-23	25.025	25.31	25.305	24.36
24-25	24.735	25.7	25.080000000000002	24.485
26-27	25.080000000000002	25.365	25.985000000000003	23.57
28-29	24.3064596895343	24.917376064096146	26.06910365548322	24.70706059088633
30-31	24.07890774018673	25.75042666398956	25.74038751129405	24.430278084529668
32-33	24.05	26.115	25.629999999999995	24.205
34-35	24.62	25.380000000000003	25.290000000000003	24.709999999999997
36-37	24.09	25.724999999999998	25.41	24.775
38-39	24.85	25.405	25.41	24.335
40-41	24.955	25.53	24.725	24.79
42-43	24.635	25.35	25.47	24.545
44-45	24.58	25.15	26.31	23.96
46-47	24.63288728512003	25.214253495714928	25.815666817019995	24.337192402145043
48-49	24.16185556568419	25.281069583713155	25.31145548465512	25.245619365947537
50-51	24.481622306717362	25.804816223067174	25.368821292775667	24.344740177439796
52-53	25.424748158171706	24.778228837768758	25.04886483235604	24.7481581717035
54-55	24.6	25.380000000000003	25.580000000000002	24.44
56-57	24.695	25.715	25.385	24.205
58-59	24.84	25.259999999999998	25.46	24.44
60-61	24.81	25.045	25.255	24.89
62-63	24.27	25.395	26.19	24.145
64-65	24.615000000000002	24.855	25.8	24.73
66-67	24.86	24.995	25.669999999999998	24.474999999999998
68-69	24.31620078148482	25.583608856827972	25.648732591924656	24.45145776976255
70-71	24.86	25.240000000000002	25.4	24.5
72-73	24.709999999999997	25.095	25.545	24.65
74-75	24.63	25.285000000000004	25.645	24.44
76-77	25.16	25.119999999999997	25.085	24.635
78-79	24.779999999999998	25.69	25.15	24.38
80-81	24.224999999999998	25.345000000000002	25.685000000000002	24.745
82-83	24.785	25.424999999999997	25.06	24.73
84-85	24.560000000000002	25.45	25.0	24.990000000000002
86-87	24.62	25.61	25.669999999999998	24.099999999999998
88-89	24.805	25.045	25.124999999999996	25.025
90-91	24.25	25.05	25.490000000000002	25.21
92-93	24.48	25.2	25.635	24.685000000000002
94-95	25.069999999999997	24.795	25.16	24.975
96-97	24.6	25.615	25.040000000000003	24.745
98-99	24.72	25.72	24.955	24.605
100-101	25.025	24.685000000000002	25.455	24.834999999999997
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	0.5
17	2.0
18	3.0
19	1.0
20	2.5
21	5.0
22	5.5
23	7.5
24	11.5
25	21.0
26	29.0
27	32.0
28	41.5
29	58.0
30	76.5
31	96.0
32	108.0
33	123.0
34	136.5
35	155.5
36	182.0
37	207.0
38	233.5
39	254.5
40	261.5
41	286.0
42	332.0
43	390.0
44	398.0
45	383.5
46	404.5
47	404.0
48	375.5
49	368.0
50	353.5
51	332.0
52	319.5
53	288.5
54	281.0
55	242.0
56	203.0
57	210.0
58	203.0
59	180.5
60	162.0
61	158.5
62	150.0
63	142.5
64	142.0
65	136.0
66	143.5
67	142.0
68	134.5
69	123.5
70	100.5
71	71.0
72	53.5
73	56.5
74	48.5
75	37.0
76	32.5
77	26.5
78	27.5
79	24.5
80	14.5
81	12.5
82	11.5
83	7.0
84	7.0
85	7.5
86	4.5
87	1.0
88	1.5
89	2.0
90	0.5
91	1.5
92	2.0
93	0.5
94	0.5
95	1.5
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.38999999999999996
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.05
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.15
30-31	0.38999999999999996
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.23500000000000001
48-49	1.27
50-51	1.375
52-53	0.23500000000000001
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.19
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	10000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.53
#Duplication Level	Percentage of total
1	95.50999999999999
2	3.4000000000000004
3	0.72
4	0.2
5	0.05
6	0.12
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.02	0.0
2	0.0	0.0	0.0	0.0	0.02	0.0
3	0.0	0.0	0.0	0.0	0.02	0.0
4	0.0	0.0	0.0	0.0	0.02	0.0
5	0.0	0.0	0.0	0.0	0.02	0.0
6	0.0	0.0	0.0	0.0	0.02	0.0
7	0.0	0.0	0.0	0.0	0.02	0.0
8	0.0	0.0	0.0	0.0	0.03	0.0
9	0.0	0.0	0.0	0.0	0.03	0.0
10-11	0.0	0.0	0.0	0.0	0.04	0.0
12-13	0.0	0.0	0.0	0.0	0.045	0.0
14-15	0.0	0.0	0.0	0.0	0.06	0.0
16-17	0.0	0.0	0.0	0.0	0.08	0.0
18-19	0.0	0.0	0.0	0.0	0.09	0.0
20-21	0.0	0.0	0.0	0.0	0.095	0.0
22-23	0.0	0.0	0.0	0.0	0.10500000000000001	0.0
24-25	0.0	0.0	0.0	0.0	0.11	0.0
26-27	0.0	0.0	0.0	0.0	0.12	0.0
28-29	0.0	0.0	0.0	0.0	0.15	0.0
30-31	0.01	0.0	0.0	0.0	0.16	0.0
32-33	0.01	0.0	0.0	0.0	0.18	0.0
34-35	0.01	0.0	0.0	0.0	0.18	0.01
36-37	0.015	0.0	0.0	0.0	0.18	0.01
38-39	0.02	0.0	0.0	0.0	0.19	0.01
40-41	0.02	0.0	0.0	0.0	0.2	0.01
42-43	0.02	0.0	0.0	0.0	0.2	0.01
44-45	0.02	0.0	0.0	0.0	0.20500000000000002	0.02
46-47	0.02	0.0	0.0	0.0	0.22499999999999998	0.02
48-49	0.02	0.0	0.0	0.0	0.245	0.025
50-51	0.02	0.0	0.0	0.0	0.27	0.05
52-53	0.02	0.0	0.0	0.0	0.28500000000000003	0.055
54-55	0.02	0.0	0.0	0.0	0.29	0.06
56-57	0.02	0.0	0.0	0.0	0.29	0.06
58-59	0.02	0.0	0.0	0.0	0.3	0.06
60-61	0.02	0.0	0.0	0.0	0.3	0.06
62-63	0.02	0.0	0.0	0.0	0.3	0.065
64-65	0.02	0.0	0.0	0.0	0.305	0.07
66-67	0.02	0.0	0.0	0.0	0.315	0.07
68-69	0.03	0.0	0.0	0.0	0.325	0.07500000000000001
70-71	0.03	0.0	0.0	0.0	0.335	0.08499999999999999
72-73	0.03	0.0	0.0	0.0	0.355	0.11
74-75	0.03	0.0	0.0	0.0	0.37	0.12
76-77	0.035	0.0	0.0	0.0	0.39	0.14500000000000002
78-79	0.04	0.0	0.0	0.0	0.4	0.165
80-81	0.04	0.0	0.0	0.0	0.4	0.17
82-83	0.04	0.0	0.0	0.0	0.405	0.18
84-85	0.04	0.0	0.0	0.0	0.415	0.18
86-87	0.04	0.0	0.0	0.0	0.435	0.21
88-89	0.04	0.0	0.0	0.0	0.45	0.21
90	0.04	0.0	0.0	0.0	0.46	0.22
>>END_MODULE