##FastQC 0.12.1 >>Basic Statistics pass #Measure Value Filename A893_fastq_origin File type Conventional base calls Encoding Illumina 1.5 Total Sequences 40664636 Total Bases 3 Gbp Sequences flagged as poor quality 0 Sequence length 76 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.2694374050219 40.0 40.0 40.0 38.0 40.0 2 39.10219535716488 40.0 40.0 40.0 38.0 40.0 3 39.15272410160022 40.0 40.0 40.0 38.0 40.0 4 39.146148412591224 40.0 40.0 40.0 38.0 40.0 5 39.144702020694346 40.0 40.0 40.0 38.0 40.0 6 39.10551667055375 40.0 40.0 40.0 38.0 40.0 7 39.075482515077724 40.0 40.0 40.0 38.0 40.0 8 39.12497050262542 40.0 40.0 40.0 38.0 40.0 9 39.033574799489166 40.0 40.0 40.0 38.0 40.0 10-11 39.03363422458767 40.0 39.5 40.0 38.0 40.0 12-13 38.883912387657915 40.0 39.0 40.0 38.0 40.0 14-15 38.90604979225684 40.0 39.0 40.0 38.0 40.0 16-17 38.78077548757599 40.0 39.0 40.0 37.0 40.0 18-19 38.63708157869654 40.0 39.0 40.0 36.0 40.0 20-21 38.86861586563815 40.0 39.0 40.0 37.5 40.0 22-23 38.761489454866876 40.0 39.0 40.0 37.0 40.0 24-25 38.71437425383569 40.0 39.0 40.0 36.5 40.0 26-27 38.48623017306733 40.0 39.0 40.0 36.0 40.0 28-29 38.489234023882574 40.0 39.0 40.0 36.0 40.0 30-31 38.38202474012063 40.0 39.0 40.0 36.0 40.0 32-33 38.3963741246817 40.0 39.0 40.0 36.0 40.0 34-35 38.317443158719044 40.0 39.0 40.0 35.5 40.0 36-37 38.339492132672724 40.0 39.0 40.0 35.5 40.0 38-39 38.275824908404445 40.0 39.0 40.0 35.0 40.0 40-41 38.190106164973415 40.0 39.0 40.0 35.0 40.0 42-43 38.06221256966373 40.0 38.0 40.0 35.0 40.0 44-45 37.852203361662944 40.0 38.0 40.0 35.0 40.0 46-47 37.61487791259216 40.0 38.0 40.0 34.0 40.0 48-49 37.49467780800989 40.0 38.0 40.0 33.0 40.0 50-51 37.14216883682421 39.0 37.0 40.0 33.0 40.0 52-53 37.00854617264987 39.0 37.0 40.0 33.0 40.0 54-55 36.80585292832819 39.0 36.0 40.0 32.5 40.0 56-57 36.516429902384964 39.0 36.0 40.0 32.0 40.0 58-59 36.03822028555721 39.0 36.0 40.0 31.0 40.0 60-61 35.784354174472384 38.5 35.0 40.0 30.5 40.0 62-63 35.37984128273028 38.0 35.0 40.0 29.5 40.0 64-65 35.053691197432585 38.0 34.0 39.5 29.0 40.0 66-67 34.43987339515347 37.5 33.5 39.0 28.5 40.0 68-69 33.93153167533579 37.0 33.0 39.0 27.0 40.0 70-71 33.11042399346695 36.0 33.0 39.0 23.0 40.0 72-73 32.156129050312906 36.0 32.0 38.5 17.5 40.0 74-75 31.694890174351986 35.5 31.0 38.0 6.0 40.0 76 28.158789617593037 33.0 24.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.1996238083416273 1 2 -0.21777435221834907 1 3 -0.21105507473679097 1 4 -0.19817191584176896 1 5 -0.2052287778083013 1 6 -0.206925738114613 1 7 -0.20482603215850048 1 8 -0.2119361497468688 1 9 -0.23299571264066543 1 10-11 -0.21473395244853322 1 12-13 0.02448455670070615 1 14-15 -0.24218428062128794 1 16-17 -0.16927529259091756 1 18-19 -0.14061266908273495 1 20-21 -0.1817861083465857 1 22-23 -0.21123323160912832 1 24-25 -0.2642309398189937 1 26-27 0.018839789241305027 1 28-29 -0.2109804049904227 1 30-31 -0.12718875908353056 1 32-33 -0.35691652565203213 1 34-35 -0.2753086339631423 1 36-37 -0.498757125365735 1 38-39 -0.5831236578202521 1 40-41 -0.6783078396017572 1 42-43 -0.8489177985544529 1 44-45 -0.9222694093419932 1 46-47 -0.9388214740436922 1 48-49 -1.1105006090119716 1 50-51 -1.2206517934847554 1 52-53 -1.3303799609541755 1 54-55 -1.4355692056892053 1 56-57 -1.5774320004233005 1 58-59 -1.5671496362810302 1 60-61 -1.9420910206110804 1 62-63 -2.0499007139874834 1 64-65 -2.2474005017271423 1 66-67 -2.2176118839718058 1 68-69 -2.1089248786010586 1 70-71 -2.41937277887736 1 72-73 -2.4060626539349386 1 74-75 -2.5507160597346648 1 76 -4.366218084936005 2 1 -0.0766491231449109 2 2 -0.07875199513553355 2 3 -0.08000711187565201 2 4 -0.09565914873248715 2 5 -0.08758512765967197 2 6 -0.1217120933410456 2 7 -0.17905078896144744 2 8 -0.12243533329379375 2 9 -0.14401938768596523 2 10-11 -0.15623871923317978 2 12-13 0.05858347174966383 2 14-15 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0.007433607125882702 113 48-49 -0.05464230001124548 113 50-51 -0.0968012404295635 113 52-53 -0.17850405066229058 113 54-55 -0.04806767366380882 113 56-57 0.05040376108724587 113 58-59 0.07914875222842 113 60-61 -0.08068871359473206 113 62-63 -0.07159876427957812 113 64-65 -0.02114406387700285 113 66-67 0.2630794839705004 113 68-69 0.32739517233560633 113 70-71 0.34158090874562674 113 72-73 0.5045563629733749 113 74-75 0.47915889544047374 113 76 -0.9786681542821292 114 1 -0.04170189472349506 114 2 -0.0733963942425504 114 3 -0.09044163078903011 114 4 -0.046677460837059925 114 5 -0.07439244743127205 114 6 -0.05294471557593283 114 7 -0.06214915719184688 114 8 -0.07020965910172805 114 9 -0.07685098735372264 114 10-11 -0.0466882685265162 114 12-13 0.17606492078711966 114 14-15 -0.06312946452755597 114 16-17 0.07456611152028358 114 18-19 0.12102142605156274 114 20-21 0.03111007279456146 114 22-23 0.03444596230592367 114 24-25 0.05749158539196486 114 26-27 0.338337092824311 114 28-29 0.13808936996687748 114 30-31 0.19927205651390523 114 32-33 0.06560655980983654 114 34-35 0.1696144952554235 114 36-37 -0.06751144108333307 114 38-39 -0.0670066846538333 114 40-41 -0.11103514439791695 114 42-43 -0.17624129239198538 114 44-45 -0.1335230669762666 114 46-47 -0.057619917037641244 114 48-49 -0.13877694049487843 114 50-51 -0.15316570744149516 114 52-53 -0.20211746028593325 114 54-55 -0.1679970063671945 114 56-57 -0.06002109538986389 114 58-59 0.05543829933299094 114 60-61 -0.12997315473860027 114 62-63 -0.18113021902483695 114 64-65 -0.19158780477359016 114 66-67 0.10373989701538022 114 68-69 0.17583470891197805 114 70-71 0.27471668057598464 114 72-73 0.4341790269740571 114 74-75 0.40002196985833294 114 76 -1.068181640387511 115 1 -0.05269185528739939 115 2 -0.08063132024588526 115 3 -0.14130604080529707 115 4 -0.09756660185757227 115 5 -0.09540809288264995 115 6 -0.09685475211531269 115 7 -0.09793615304478465 115 8 -0.10426075106222044 115 9 -0.08900775736512401 115 10-11 -0.08711964705532438 115 12-13 0.14493477752970563 115 14-15 -0.10364797224207933 115 16-17 0.007023525849106704 115 18-19 0.07740636906203946 115 20-21 -0.02378138962508558 115 22-23 0.024497681270538862 115 24-25 -0.009629080181213112 115 26-27 0.196396230556509 115 28-29 0.07938419975589284 115 30-31 0.18766482731288647 115 32-33 0.028138324821888716 115 34-35 0.14364063731189702 115 36-37 -0.07318715434932699 115 38-39 -0.1156147476550089 115 40-41 -0.13538140046627944 115 42-43 -0.1872578743526816 115 44-45 -0.16420138611248802 115 46-47 -0.10283070607391664 115 48-49 -0.2040655169436718 115 50-51 -0.17649727083951205 115 52-53 -0.2674338618337089 115 54-55 -0.23035933129715858 115 56-57 -0.1457101841366253 115 58-59 0.03011905887534283 115 60-61 -0.12182320188152573 115 62-63 -0.19806182330000865 115 64-65 -0.328368764819956 115 66-67 0.07538450557827758 115 68-69 0.1981670247340972 115 70-71 0.1664405240395581 115 72-73 0.49426899927536283 115 74-75 0.34085404213958626 115 76 -1.1679134169620653 116 1 -0.04458824705122311 116 2 -0.06854966338856627 116 3 -0.0788602473164488 116 4 -0.08018030662636022 116 5 -0.09307430485449686 116 6 -0.08042024978512785 116 7 -0.08874219338007094 116 8 -0.09968022750641126 116 9 -0.10841572248926923 116 10-11 -0.08815711823751116 116 12-13 0.1364161378763029 116 14-15 -0.09481722017935823 116 16-17 0.025753831926110138 116 18-19 0.06961830663629343 116 20-21 -4.5928137336659347E-4 116 22-23 -0.005124551273596012 116 24-25 0.0026190168256121638 116 26-27 0.28654119838575554 116 28-29 0.09294687712680627 116 30-31 0.2046415006840263 116 32-33 0.04744673491253337 116 34-35 0.14101416418455415 116 36-37 -0.05243831904315499 116 38-39 -0.05951236278896488 116 40-41 -0.14570336731655686 116 42-43 -0.17675858954795842 116 44-45 -0.16604106999966461 116 46-47 -0.007057151281699703 116 48-49 -0.09611826337541629 116 50-51 -0.08598638204082931 116 52-53 -0.09933520207547986 116 54-55 -0.09723395385206857 116 56-57 0.05753535547665223 116 58-59 0.15431300265064607 116 60-61 -0.0677250729727703 116 62-63 -0.09267634790515444 116 64-65 -0.10440930795495262 116 66-67 0.21973833576520008 116 68-69 0.3692913497275754 116 70-71 0.34300203801928575 116 72-73 0.6708099271631411 116 74-75 0.5216674902328329 116 76 -1.107038565170189 117 1 -0.03822527985128943 117 2 -0.06326591082486743 117 3 -0.052771577620724486 117 4 -0.07685997897895191 117 5 -0.08195476500532095 117 6 -0.1522315082745891 117 7 -0.35179277095998884 117 8 -0.16964121776776153 117 9 -0.1976931923893872 117 10-11 -0.12828888425627127 117 12-13 0.11584460412644404 117 14-15 -0.13511206362989725 117 16-17 0.01481289452639345 117 18-19 0.044531540718743656 117 20-21 -0.007282539308036462 117 22-23 -0.020369663292868267 117 24-25 -0.08058784215534587 117 26-27 -0.05165052173244078 117 28-29 0.008176349414995343 117 30-31 0.14972260785227576 117 32-33 -0.00803062151842937 117 34-35 0.12712898354161695 117 36-37 -0.11825137505660877 117 38-39 -0.14272520375769204 117 40-41 -0.15720492007167763 117 42-43 -0.2317114980734516 117 44-45 -0.24414765101946756 117 46-47 -0.11331998176576263 117 48-49 -0.2064703113786024 117 50-51 -0.20215124966338038 117 52-53 -0.24179297923525667 117 54-55 -0.22299054264564688 117 56-57 -0.1747373038637292 117 58-59 -0.06759030701850577 117 60-61 -0.1997647419827686 117 62-63 -0.345509430684686 117 64-65 -0.3026252328846937 117 66-67 -0.0017655586918223776 117 68-69 0.05233770810595928 117 70-71 0.010686126092281256 117 72-73 0.3103024826004841 117 74-75 0.1944231881184102 117 76 -1.5482960300927289 118 1 -0.04595443927542675 118 2 -0.05926285288127531 118 3 -0.06916164770481004 118 4 -0.06863753235417391 118 5 -0.06375183460185951 118 6 -0.0988300581981747 118 7 -0.10791079085911548 118 8 -0.09479374930680251 118 9 -0.1076455645567691 118 10-11 -0.10234396642955801 118 12-13 0.13456701835922757 118 14-15 -0.13349254295480506 118 16-17 -0.03176498622374879 118 18-19 0.05076380437497363 118 20-21 -0.021275097739881232 118 22-23 -0.01814738412775796 118 24-25 -0.010337339845747806 118 26-27 0.22271493324031866 118 28-29 0.03139379598005121 118 30-31 0.16064063198850675 118 32-33 -0.04639160528932251 118 34-35 0.07114763984146322 118 36-37 -0.1579638942658832 118 38-39 -0.1737259568658942 118 40-41 -0.270728184957278 118 42-43 -0.30787101734396316 118 44-45 -0.29998978496583106 118 46-47 -0.1560404526178374 118 48-49 -0.27821326553591064 118 50-51 -0.32077887735619726 118 52-53 -0.3040597782178409 118 54-55 -0.356713575240434 118 56-57 -0.2515374636576837 118 58-59 -0.1389121055843816 118 60-61 -0.291203392983725 118 62-63 -0.36183416373675925 118 64-65 -0.3243557582962211 118 66-67 -0.18158749000709662 118 68-69 -0.029831260308185392 118 70-71 -0.06390306332625073 118 72-73 0.2178608908090638 118 74-75 0.06930314533317272 118 76 -1.6207006380346094 119 1 -0.09102384062192925 119 2 -0.08268476700347804 119 3 -0.08508982754165828 119 4 -0.12230769275649322 119 5 -0.11179837253137492 119 6 -0.11673905800601858 119 7 -0.12884560467013273 119 8 -0.12631419352364048 119 9 -0.13846766601447058 119 10-11 -0.12604602123528252 119 12-13 0.07727863833009252 119 14-15 -0.17737657404487805 119 16-17 -0.0725603795436669 119 18-19 -0.048060243844055606 119 20-21 -0.10303527497065801 119 22-23 -0.12271286269847081 119 24-25 -0.10142885204157182 119 26-27 0.19148769827879875 119 28-29 -0.0318760430503815 119 30-31 0.004190702564280002 119 32-33 -0.11418371429088126 119 34-35 0.01881678146873611 119 36-37 -0.3004556292599503 119 38-39 -0.2600723336786217 119 40-41 -0.3544653553306034 119 42-43 -0.37928021882323293 119 44-45 -0.4258326647289934 119 46-47 -0.33009398024342573 119 48-49 -0.41598778347689347 119 50-51 -0.4219552377244824 119 52-53 -0.5075052732190528 119 54-55 -0.4708052009249144 119 56-57 -0.4150441530004727 119 58-59 -0.3132055658168724 119 60-61 -0.5603532818201771 119 62-63 -0.5850392713214916 119 64-65 -0.6354600277096196 119 66-67 -0.5649555678495162 119 68-69 -0.44816289922946595 119 70-71 -0.40084237602659556 119 72-73 -0.14987014544639266 119 74-75 -0.4678110304309975 119 76 -1.989007470612517 120 1 -0.14631425891799665 120 2 -0.09269908811679528 120 3 -0.1376926988405529 120 4 -0.15391613666165682 120 5 -0.1601876332071015 120 6 -0.13448634820233707 120 7 -0.14937253486231583 120 8 -0.14257913682111933 120 9 -0.1569308702348664 120 10-11 -0.14990725043314512 120 12-13 0.057502412141069215 120 14-15 -0.20550379001748098 120 16-17 -0.09402777496404724 120 18-19 -0.05112297360432905 120 20-21 -0.07254504225146263 120 22-23 -0.05776293958068379 120 24-25 -0.0487884367314777 120 26-27 0.23086113125430785 120 28-29 -0.020493776594825874 120 30-31 0.09202126516929354 120 32-33 -0.07340189362823679 120 34-35 0.07757653811211895 120 36-37 -0.166615343593584 120 38-39 -0.20679506963742966 120 40-41 -0.2585785010033277 120 42-43 -0.260382320607043 120 44-45 -0.314561403955139 120 46-47 -0.1921104579032047 120 48-49 -0.218663682344598 120 50-51 -0.25961933649387703 120 52-53 -0.24851772962486507 120 54-55 -0.2597515840311573 120 56-57 -0.15022649431171686 120 58-59 -0.034994311134354916 120 60-61 -0.20929038521801857 120 62-63 -0.20634958363905298 120 64-65 -0.19626276348233773 120 66-67 0.09812212393585895 120 68-69 0.04226016870102711 120 70-71 0.20130920247257222 120 72-73 0.38765353984494055 120 74-75 0.2018798891227256 120 76 -1.776136736809928 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 111691.0 3 9295.0 4 7974.0 5 9549.0 6 11967.0 7 10601.0 8 15122.0 9 16308.0 10 20082.0 11 23947.0 12 24874.0 13 32204.0 14 33548.0 15 38958.0 16 46534.0 17 47581.0 18 59421.0 19 62426.0 20 73069.0 21 85438.0 22 90400.0 23 109956.0 24 121695.0 25 146268.0 26 175765.0 27 197394.0 28 242319.0 29 283649.0 30 342784.0 31 422890.0 32 540813.0 33 762443.0 34 1097813.0 35 1748598.0 36 3022306.0 37 5261452.0 38 1.0382856E7 39 1.4973946E7 40 700.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.4222971354645 31.817207476788823 31.78336063809094 18.977134749655736 2 19.15066847099504 33.443772680995096 29.560874386215286 17.84468446179458 3 18.826720939026604 31.366550559068845 31.24031567329597 18.566412828608584 4 19.016091611871563 30.85798689747572 31.072920944027654 19.05300054662506 5 19.069566536778726 30.902818881275795 31.565996809424362 18.461617772521123 6 18.9765084102845 31.209400423444052 30.82732191745362 18.986769248817833 7 19.136595646614346 31.047493917011632 31.423457400143196 18.392453036230826 8 18.724869407798796 31.615411700926455 31.063178423463267 18.596540467811483 9 18.627917188785933 30.999418274105906 31.662336795106388 18.710327742001773 10-11 18.629578379675962 31.209073220378837 31.401939658617916 18.75940874132728 12-13 18.52640463093867 31.1717399676934 31.516750961488 18.785104439879927 14-15 18.504590189453328 31.224085733531037 31.625588576365814 18.64573550064982 16-17 18.57341776865478 31.169840550175117 31.538645776144147 18.718095905025955 18-19 18.750326336510447 30.99252756580263 31.38579803924663 18.87134805844029 20-21 18.707485276983515 30.951333221526184 31.375758906997337 18.965422594492964 22-23 18.957627604952034 30.941643569654246 31.23201374193837 18.86871508345535 24-25 18.816457764943813 30.94840882087248 31.262841104421675 18.972292309762032 26-27 18.97344624855493 30.922407387855248 31.134682958123143 18.969463405466676 28-29 18.98938260188362 30.93787990241787 31.019798049766674 19.05293944593184 30-31 19.01785850010284 30.823460414798603 30.98459631373544 19.174084771363116 32-33 19.02147438097743 30.931887686136967 30.977017837109226 19.069620095776372 34-35 18.953284266868838 30.85969224557323 31.003943291647335 19.183080195910602 36-37 19.007883081197384 30.894335328186656 30.996239112833006 19.10154247778295 38-39 18.977158103366943 30.88779313949362 31.003838405190397 19.13121035194904 40-41 19.04187716523952 30.877016418264546 30.9915476634663 19.089558753029632 42-43 19.04767638425199 30.840693847450666 31.05458402769738 19.05704574059996 44-45 19.002509154734835 30.937769154714168 31.008656145980247 19.051065544570754 46-47 19.10908160384539 30.960787465481825 30.884351296509553 19.045779634163225 48-49 19.101883886498978 30.92343117807485 30.829275716128297 19.14540921929787 50-51 19.240146504719124 30.841911231727032 30.779898710790903 19.138043552762937 52-53 19.177031135975973 30.94767250730126 30.86375949938353 19.011536857339237 54-55 19.155017881836212 30.898311082954194 30.826800223482664 19.11987081172693 56-57 19.298519065564523 30.88375397140048 30.748082424063934 19.069644538971065 58-59 19.378160684048957 30.893627869365716 30.653564592918787 19.074646853666543 60-61 19.413553441262167 30.93534263126323 30.582605786744267 19.06849814073034 62-63 19.394626963899483 30.9145357103806 30.555814157627314 19.135023168092598 64-65 19.48303091316571 30.98917189154557 30.50973406624738 19.01806312904134 66-67 19.461978330522257 30.897057731887944 30.516753017860026 19.124210919729766 68-69 19.600489540801302 30.869734899654645 30.377816586383343 19.15195897316071 70-71 19.666654859403828 30.881970265305252 30.360575332611333 19.090799542679584 72-73 19.68351191260897 30.792066952288 30.3487902600848 19.175630875018225 74-75 19.708477950260466 30.881170778757376 30.34309207347372 19.067259197508434 76 20.640761949114385 30.297896962960568 29.898906533421805 19.162434554503246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3490.0 1 2601.5 2 1617.5 3 1625.0 4 1728.0 5 1962.5 6 2559.0 7 3502.0 8 4083.0 9 6043.0 10 10118.5 11 15419.5 12 18605.0 13 25775.5 14 45841.5 15 79392.5 16 128326.5 17 156605.0 18 197142.5 19 291087.0 20 415727.0 21 486960.0 22 570907.5 23 747549.5 24 938005.0 25 1035766.0 26 1131660.5 27 1333572.5 28 1527326.5 29 1615063.0 30 1668285.0 31 1761024.0 32 1833996.5 33 1867452.0 34 1894369.5 35 1941627.0 36 1964764.0 37 1967561.0 38 1981718.0 39 1999155.0 40 2010252.5 41 2012915.0 42 2007760.0 43 1978914.0 44 1897723.0 45 1763066.0 46 1680754.0 47 1587884.5 48 1389519.5 49 1179089.5 50 1074155.0 51 977648.0 52 796277.0 53 639824.5 54 568236.0 55 513605.5 56 412362.0 57 315800.5 58 265852.0 59 221676.5 60 145788.0 61 93958.0 62 73841.0 63 60862.5 64 39616.0 65 26037.0 66 17667.5 67 14609.0 68 12387.5 69 8868.0 70 6690.0 71 5810.0 72 5188.0 73 4184.0 74 3413.0 75 3024.0 76 2789.5 77 2342.5 78 1968.5 79 1807.0 80 1668.5 81 1463.5 82 1317.5 83 1238.0 84 1127.5 85 996.5 86 901.5 87 827.0 88 788.5 89 687.0 90 595.0 91 501.5 92 437.0 93 386.0 94 288.5 95 230.0 96 218.0 97 233.0 98 264.0 99 281.5 100 283.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09949185331451141 2 0.03137861605351638 3 0.013552316071389401 4 0.005889638358007189 5 0.01664591317133639 6 0.012765391530862345 7 0.015163052240280719 8 0.010532493147116823 9 0.05356742895719022 10-11 0.017130363591598363 12-13 0.0545019018490661 14-15 0.016947157722006907 16-17 0.01587128432675507 18-19 0.035646452116281084 20-21 0.013129344130856108 22-23 0.021997000046920373 24-25 0.012703913051133667 26-27 0.06845136889950276 28-29 0.014158493881514151 30-31 0.02415366511580234 32-33 0.021859288252328142 34-35 0.044601407473560076 36-37 0.016417213226745716 38-39 0.022006836603676964 40-41 0.020857189032750717 42-43 0.026631247848867994 44-45 0.029268674629228206 46-47 0.03696578029125848 48-49 0.022137170980701757 50-51 0.062124003765827385 52-53 0.07302782693050541 54-55 0.055470802689590044 56-57 0.0676828879028943 58-59 0.141217297506364 60-61 0.10154400496785462 62-63 0.09893116957938589 64-65 0.11368723428386276 66-67 0.1193198925966041 68-69 0.18686137999612243 70-71 0.16760017229712815 72-73 0.39823914813844635 74-75 0.19914600981550654 76 0.13498215009228168 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 4.0664636E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.93379271647123 #Duplication Level Percentage of total 1 54.3232157515976 2 16.154049087779384 3 4.546599775033058 4 1.9226479604255238 5 1.5622074161752815 6 1.308761830076954 7 1.1557796444770687 8 1.059973086448158 9 0.8436807485821315 >10 10.05919039062838 >50 4.162985527640785 >100 2.2058387204599534 >500 0.20331569705049346 >1k 0.44309435629619953 >5k 0.0 >10k+ 0.048660007329128015 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG 1 0.001050052433765791 1.1312040270076436E-4 2.4591391891470513E-6 4.918278378294103E-6 0.014085949275434311 8.484030202557328E-4 2 0.0016820512053765833 2.139451094557935E-4 2.4591391891470513E-6 7.377417567441155E-6 0.017287748499703774 0.0010598889905223791 3 0.0022255209661780815 3.1476981621082257E-4 4.918278378294103E-6 7.377417567441155E-6 0.020388723017218204 0.0013525265540308784 4 0.0027001348296834624 4.401859148573222E-4 4.918278378294103E-6 1.475483513488231E-5 0.023312639513114047 0.00172631571078123 5 0.0031895035283237257 5.606837351255277E-4 4.918278378294103E-6 1.475483513488231E-5 0.026477551649546304 0.003056710012109785 6 0.0036100163296678717 6.71344998637145E-4 7.377417567441155E-6 1.967311351317641E-5 0.029494915434629735 0.004269065632359281 7 0.004032988270201165 7.549557310681448E-4 9.836556756588205E-6 2.7050531080617566E-5 0.03254424802917208 0.00470433326883831 8 0.004483010741815075 8.238116283642622E-4 9.836556756588205E-6 2.7050531080617566E-5 0.03541652260209584 0.00511255037423672 9 0.004800239697215045 8.803718297146444E-4 1.475483513488231E-5 3.442794864805872E-5 0.038101902596644414 0.009568510584971178 10 0.005203538524235161 9.443094486324678E-4 1.721397432402936E-5 3.442794864805872E-5 0.04089794385470461 0.012015354078172493 11 0.005624051325579307 0.0010107062067394382 1.967311351317641E-5 3.688708783720577E-5 0.043607915241144664 0.025961132419825422 12 0.006037186709356011 0.0011139900526836142 2.2132252702323464E-5 3.934622702635282E-5 0.04625394900866689 0.028678481223832916 13 0.006639675810697039 0.0012074373418712024 2.7050531080617566E-5 4.426450540464693E-5 0.04900572576132244 0.030830228014336585 14 0.006954445626907862 0.001295966352680496 3.196880945891167E-5 4.672364459379398E-5 0.05168618747749273 0.03174502779269929 15 0.007726615332300036 0.0013918727810572312 3.442794864805872E-5 5.164192297208808E-5 0.054342057801771544 0.03229341583187908 16 0.008038926009321712 0.0014681060959207898 3.442794864805872E-5 5.164192297208808E-5 0.05681595182605348 0.0335279037048309 17 0.008567640934988328 0.0015590942459192307 3.934622702635282E-5 5.410106216123513E-5 0.059326732938172616 0.0343222056629254 18 0.008956184926873561 0.0016353275607827894 4.180536621549988E-5 5.656020135038218E-5 0.06176619901380649 0.03504519258453463 19 0.009425880512000648 0.00172631571078123 4.9182783782941033E-5 5.901934053952924E-5 0.0641859919759272 0.035650140825064805 20 0.021037935763153024 0.0018369769742928475 5.164192297208808E-5 6.393761891782334E-5 0.0665541430150758 0.03623787509127095 21 0.021738790432059934 0.0019279651242912884 5.410106216123513E-5 6.63967581069704E-5 0.06908951551908642 0.037233826462875506 22 0.022053560248270758 0.0020287898310463173 5.901934053952924E-5 6.63967581069704E-5 0.07162242888390788 0.03784123384259483 23 0.022530633250965285 0.0021222371202339056 6.393761891782334E-5 7.13150364852645E-5 0.0738036853446813 0.03836257135069401 24 0.022938850356363696 0.002188633878340876 6.63967581069704E-5 7.377417567441154E-5 0.07611035790410124 0.03883226693582109 25 0.023433137333382252 0.0022599489148261405 7.377417567441154E-5 8.11515932418527E-5 0.07831374661757701 0.03931425821689391 26 0.024148746837424045 0.0023558553432028754 7.377417567441154E-5 8.60698716201468E-5 0.08040155578916285 0.03976673982769697 27 0.025474222860374308 0.0024468434932013164 7.623331486355859E-5 8.852901080929385E-5 0.08284839928236416 0.04030037303174188 28 0.028405516773837593 0.002530454225632316 7.623331486355859E-5 8.852901080929385E-5 0.08498293209854381 0.04083400623578679 29 0.030722025890014115 0.002641115489143933 7.623331486355859E-5 8.852901080929385E-5 0.0871568111417498 0.04138485341415573 30 0.03363610582915337 0.0027640724486012857 8.11515932418527E-5 9.09881499984409E-5 0.08946102456198059 0.041888976947930874 31 0.03819043160745371 0.0028771928513020503 8.11515932418527E-5 9.5906428376735E-5 0.09176769712140052 0.042498843466839346 32 0.04314067879520672 0.002958344444543903 8.60698716201468E-5 9.836556756588207E-5 0.09403748259298325 0.04296607991277728 33 0.049583623470772 0.0030321186202183145 9.09881499984409E-5 9.836556756588207E-5 0.09627284011591791 0.04348004000330902 34 0.057428277484151094 0.0031009745175144317 9.344728918758795E-5 1.0328384594417617E-4 0.09851065677804173 0.044035805460056254 35 0.06759681803127415 0.003147698162108226 9.344728918758795E-5 1.0820212432247027E-4 0.10059600681043844 0.044594030055992634 36 0.0800277666324125 0.0032140949202151964 9.836556756588207E-5 1.1312040270076436E-4 0.10276742671445528 0.04523094710598172 37 0.09524491993485446 0.003292787374267902 1.0082470675502912E-4 1.2049782026820553E-4 0.10501262079414654 0.04586540501678166 38 0.1136392810696744 0.0033714798283206076 1.0082470675502912E-4 1.2295695945735256E-4 0.10739798580761918 0.046526913458662215 39 0.13595842835037303 0.0034575496999407545 1.0328384594417617E-4 1.278752378356467E-4 0.10969482181028253 0.047301542303243536 40 0.1617695532796605 0.003533783014804313 1.0574298513332322E-4 1.3033437702479373E-4 0.11186378257511023 0.04861472263024806 41 0.18896517357243772 0.0036100163296678717 1.1312040270076436E-4 1.3033437702479373E-4 0.11414586374263869 0.049576246053204556 42 0.21868387067327985 0.003720677593179489 1.2295695945735256E-4 1.327935162139408E-4 0.11626564172368345 0.05027464158292232 43 0.2506698941065155 0.003833797995880253 1.2541609864649963E-4 1.327935162139408E-4 0.11847148957634836 0.05098533280858582 44 0.2854494996586223 0.003964132372905047 1.278752378356467E-4 1.3525265540308782E-4 0.12046339231955747 0.0517058605910059 45 0.32402847525796125 0.004035447409390312 1.278752378356467E-4 1.4017093378138192E-4 0.1226864541465464 0.05238950128558879 46 0.3652362706504984 0.004106762445875576 1.3033437702479373E-4 1.4017093378138192E-4 0.12496607617488571 0.05320839463557475 47 0.4108705165835002 0.004187914039117429 1.3033437702479373E-4 1.475483513488231E-4 0.12699486600593204 0.05395105467069716 48 0.46063365721507993 0.00426168821479184 1.3525265540308782E-4 1.5000749053797015E-4 0.12922776438967756 0.054691255566630424 49 0.5155044299425181 0.0043207075553313695 1.4017093378138192E-4 1.5984404729455835E-4 0.13142131654639672 0.05547818010715748 50 0.5742557243104304 0.004389563452627487 1.4017093378138192E-4 1.6476232567285245E-4 0.13361732784230504 0.056208544446334156 51 0.6360391372985609 0.004519897829652281 1.42630072970529E-4 1.672214648619995E-4 0.13579120688551105 0.05698563243010463 52 0.6977266438583146 0.004588753726948398 1.475483513488231E-4 1.7459888242944065E-4 0.13805361493952634 0.057779934388199124 53 0.7596846557288746 0.004677282737757692 1.5492576891626425E-4 1.7951716080773477E-4 0.14022749398273232 0.05855702237196959 54 0.8238558928696669 0.004760893470188691 1.5984404729455835E-4 1.7951716080773477E-4 0.14236694507729025 0.059329192077361766 55 0.8892739135793568 0.004839585924241397 1.6476232567285245E-4 1.868945783751759E-4 0.14466624021914273 0.06005709727734929 56 0.9598684222822012 0.00489614612559178 1.6476232567285245E-4 1.8935371756432297E-4 0.14690897515964485 0.06092763255030735 57 1.0328187863282485 0.004942869770185573 1.6968060405114655E-4 1.9427199594261707E-4 0.1494984487258167 0.061746525900293316 58 1.108968490459376 0.0050018891107251025 1.7459888242944065E-4 1.9673113513176413E-4 0.17170693474300372 0.0625506644151444 59 1.191430804889044 0.0050805815647778085 1.7951716080773477E-4 2.0164941351005823E-4 0.19215959537913976 0.06340398571377844 60 1.2788409073672762 0.00515435574045222 1.7951716080773477E-4 2.0410855269920527E-4 0.19994277091279017 0.06429173496106051 61 1.36661988072388 0.005223211637748338 1.819762999968818E-4 2.1148597026664643E-4 0.2172231419949265 0.06518686162591004 62 1.453225844687261 0.005284690117477014 1.8443543918602887E-4 2.139451094557935E-4 0.22015935418676807 0.0661360893529208 63 1.5404121654992804 0.005353546014773131 1.8443543918602887E-4 2.139451094557935E-4 0.22258406542726708 0.06698941065155482 64 1.62990515887072 0.005415024494501808 1.868945783751759E-4 2.139451094557935E-4 0.22473581221777075 0.06798782116234853 65 1.720777729327271 0.005481421252608778 1.9673113513176413E-4 2.1640424864494053E-4 0.2271211772312434 0.0690206596217903 >>END_MODULE